中华老年心脑血管病杂志
中華老年心腦血管病雜誌
중화노년심뇌혈관병잡지
CHINESE JOURNAL OF GERIATRIC CARDIOVASCULAR AND CEREBROVASCULAR DISEASES
2014年
6期
564-567
,共4页
苏强%李浪%黄伟强%周游%王江友%伟明
囌彊%李浪%黃偉彊%週遊%王江友%偉明
소강%리랑%황위강%주유%왕강우%위명
心绞痛 ,不稳定型%CD4阳性T淋巴细胞%细胞死亡%降血脂药%羟甲基戊二酰基CoA还原酶抑制剂
心絞痛 ,不穩定型%CD4暘性T淋巴細胞%細胞死亡%降血脂藥%羥甲基戊二酰基CoA還原酶抑製劑
심교통 ,불은정형%CD4양성T림파세포%세포사망%강혈지약%간갑기무이선기CoA환원매억제제
angina,unstable%CD4-positive T-lymphocytes%cell death%antilipemic agents%hydroxy-methylglutaryl-CoA reductase inhibitors
目的：探讨负荷剂量瑞舒伐他汀对不稳定性心绞痛患者PCI围术期CD4T淋巴细胞程序性细胞死亡因子4（PDCD4）表达的影响。方法选择入住我院不稳定性心绞痛患者92例，随机分为负荷剂量瑞舒伐他汀组（负荷剂量组，术前2d给予20mg/d，术后10mg/d）46例和常规剂量瑞舒伐他汀组（常规剂量组，术前2d、术后均10mg/d）46例。采用免疫磁珠法筛选出CD4T淋巴细胞，荧光定量PCR检测PDCD4mRNA表达；蛋白免疫印迹法检测PDCD4蛋白相对表达量；酶联免疫法检测血清TNF-α水平。结果与PCI前比较，负荷剂量组PCI后PDCD4mRNA和PDCD4蛋白相对表达量明显降低（P＜0．05）。PCI后常规剂量组较负荷剂量组TNF-α水平明显升高[（14．62±2．45）ng/Lvs（9．51±1．93）ng/L，P＜0．05]。结论负荷剂量瑞舒伐他汀可以通过抑制CD4T淋巴细胞PDCD4的表达，从而减少PCI术后心肌的炎性反应。
目的：探討負荷劑量瑞舒伐他汀對不穩定性心絞痛患者PCI圍術期CD4T淋巴細胞程序性細胞死亡因子4（PDCD4）錶達的影響。方法選擇入住我院不穩定性心絞痛患者92例，隨機分為負荷劑量瑞舒伐他汀組（負荷劑量組，術前2d給予20mg/d，術後10mg/d）46例和常規劑量瑞舒伐他汀組（常規劑量組，術前2d、術後均10mg/d）46例。採用免疫磁珠法篩選齣CD4T淋巴細胞，熒光定量PCR檢測PDCD4mRNA錶達；蛋白免疫印跡法檢測PDCD4蛋白相對錶達量；酶聯免疫法檢測血清TNF-α水平。結果與PCI前比較，負荷劑量組PCI後PDCD4mRNA和PDCD4蛋白相對錶達量明顯降低（P＜0．05）。PCI後常規劑量組較負荷劑量組TNF-α水平明顯升高[（14．62±2．45）ng/Lvs（9．51±1．93）ng/L，P＜0．05]。結論負荷劑量瑞舒伐他汀可以通過抑製CD4T淋巴細胞PDCD4的錶達，從而減少PCI術後心肌的炎性反應。
목적：탐토부하제량서서벌타정대불은정성심교통환자PCI위술기CD4T림파세포정서성세포사망인자4（PDCD4）표체적영향。방법선택입주아원불은정성심교통환자92례，수궤분위부하제량서서벌타정조（부하제량조，술전2d급여20mg/d，술후10mg/d）46례화상규제량서서벌타정조（상규제량조，술전2d、술후균10mg/d）46례。채용면역자주법사선출CD4T림파세포，형광정량PCR검측PDCD4mRNA표체；단백면역인적법검측PDCD4단백상대표체량；매련면역법검측혈청TNF-α수평。결과여PCI전비교，부하제량조PCI후PDCD4mRNA화PDCD4단백상대표체량명현강저（P＜0．05）。PCI후상규제량조교부하제량조TNF-α수평명현승고[（14．62±2．45）ng/Lvs（9．51±1．93）ng/L，P＜0．05]。결론부하제량서서벌타정가이통과억제CD4T림파세포PDCD4적표체，종이감소PCI술후심기적염성반응。
Objective To study the effect of loading dose rosuvastatin on PDCD4 expression in un-stable angina pectoris patients undergoing perioperative PCI .Methods Ninety-two unstable angi-na pectoris patients admitted to our hospital were randomly divided into loading dose rosuvastatin treatment group and conventional dose rosuvastatin treatment group (46 in each group) .Patients in loading dose rosuvastatin treatment group were given 20 mg rosuvastatin daily before PCI and 10 mg rosuvastatin daily after PCI while those in conventional dose rosuvastatin treatment group were given 10 mg rosuvastatin daily before and after PCI .Circulating CD4 T lymphocytes were isolated using the magnetic cell sorting system .Expression of PDCD4 mRNA and protein was de-tected by RFQ-PCR and Western blot ,respectively .Serum TNF-αlevel was measured by ELISA . Results The expression levels of PDCD4 mRNA and protein were significantly lower in loading dose rosuvastatin treatment group after PCI than before PCI (P<0 .05) .The serum TNF-α level was significantlt higher in conventional dose rosuvastatin treatment group than in loading dose ro-suvastatin treatment group (14 .62 ± 2 .45 ng/L vs 9 .51 ± 1 .93 ng/L ,P< 0 .05) .Conclusion Loading dose rosuvastatin treatment can reduce the inflammatory reactions in myocardium after PCI by inhibiting the PDCD4 expression in CD4 T lymphocytes .
