CAJ | 학술논문

目的：比较适用于两种设备的比浊法与化学发光法在检测同一组样品中血清蛋白含量的差异。方法：使用全自动生化分析仪Synchron CX4PRO/Beckman与Beckman DXI800免疫分析系统，应用免疫比浊法与化学发光法分别检测176例血清铁蛋白含量。对两组检测结果进行两组均数比较的t检验，配对比较的t检验，对两种方法所判断的铁缺乏情况进行卡方检验，并对两种设备所用的两种方法的准确性进行分析。结果：适用于两种设备的两方法间的差异有统计学意义，两种方法的相关系数仅为0.585；二者判断铁缺乏的差异有统计学意义；比浊法的灵敏度、阳性似然比、阴性似然比和约登指数均较高于化学发光法，而化学发光法的特异度高于比浊法；两方法判断铁缺乏情况的一致性分析Kappa值显示，两种方法的一致性不理想，只具有中度一致性。结论：适用于两种设备的两种方法对同一组样品的检测结果间存在统计学差异，两方法判断铁缺乏情况只具有中度一致性，世界卫生组织(WHO)应对检测血清铁蛋白的设备与方法进行标准化统一。
목적：비교괄용우량충설비적비탁법여화학발광법재검측동일조양품중혈청단백함량적차이。방법：사용전자동생화분석의Synchron CX4PRO/Beckman여Beckman DXI800면역분석계통，응용면역비탁법여화학발광법분별검측176례혈청철단백함량。대량조검측결과진행량조균수비교적t검험，배대비교적t검험，대량충방법소판단적철결핍정황진행잡방검험，병대량충설비소용적량충방법적준학성진행분석。결과：괄용우량충설비적량방법간적차이유통계학의의，량충방법적상관계수부위0.585；이자판단철결핍적차이유통계학의의；비탁법적령민도、양성사연비、음성사연비화약등지수균교고우화학발광법，이화학발광법적특이도고우비탁법；량방법판단철결핍정황적일치성분석Kappa치현시，량충방법적일치성불이상，지구유중도일치성。결론：괄용우량충설비적량충방법대동일조양품적검측결과간존재통계학차이，량방법판단철결핍정황지구유중도일치성，세계위생조직(WHO)응대검측혈청철단백적설비여방법진행표준화통일。
Objective: To compare the difference of turbidimetric immunoassay and chemiluminescence were appropriate for different equipment on the one group serum sample. Methods: Turbidimetric immunoassay and chemiluminescence (turbidimetric immunoassay used fully automatic biochemical analyser Synchron CX4PRO/Beckman, chemiluminescence assay used Beckman DXI800 immunoassay analytic system) were applied to detect 176 serum, respectively. Used independent sample t test, paired compared t test and chi-square test to compare the difference between the two methods and analyzed the accuracy of the two methods. Results:There is significant difference between the two method were appropriate for different equipment, the relative coefficient is 0.585; the sensitivity, positive likelihood ratio, negative likelihood ratio,Youden’s index of turbidimetric immunoassay is more accuracy than chemiluminescence;however, the specificity of chemiluminescence is better than turbidimetric immunoassay. And the kappa is not undesirability. Conclusion: there is significant difference between the two method were appropriate for different equipment, and WHO should draw up the uniform and standard equipment and detection method on measure the serum ferritin.