CAJ | 학술논문

Squamosa-promoter binding protein (SBP)-box基因是植物特有的一类转录因子,广泛参与植物生长发育,其部分成员受miR156调控。文章克隆了小麦(Triticum aestivum) tae-MIR156前体基因,转录后能够形成茎环结构。小麦10个SBP-box基因中,仅TaSPL3和TaSPL17在编码区存在tae-miR156识别位点。SPL17在普通小麦的 A 基因组供体种乌拉尔图小麦(Triticum urartu, AA) UR209和 B 基因组供体种拟斯卑尔脱山羊草(Aegilops speltoides, BB) Y2001中均为多拷贝(SPL17-A1、SPL17-A2和SPL17-A3；SPL17-B1、SPL17-B2和SPL17-B3),在D基因组供体种粗山羊草(Aegilops tauschii, DD) Ae38中仅检测到一种序列(SPL17-D)； SPL17-A2与SPL17-B2, SPL17-A3与 SPL17-B3、SPL17-D两两之间序列的一致性程度均大于99%,且与普通小麦(中国春、衡观35和双丰收)的 TaSPL17序列具有较高的一致性,提示它们可能来源于共同的祖先基因,并且在进化过程中高度保守。靶基因 TaSPL17中的 tae-miR156识别位点非常保守,在根据单株穗数和基因型多样性挑选的 SubP1和SubP2群体中均未检测到tae-miR156识别位点存在变异碱基。
Squamosa-promoter binding protein (SBP)-box기인시식물특유적일류전록인자,엄범삼여식물생장발육,기부분성원수miR156조공。문장극륭료소맥(Triticum aestivum) tae-MIR156전체기인,전록후능구형성경배결구。소맥10개SBP-box기인중,부TaSPL3화TaSPL17재편마구존재tae-miR156식별위점。SPL17재보통소맥적 A 기인조공체충오랍이도소맥(Triticum urartu, AA) UR209화 B 기인조공체충의사비이탈산양초(Aegilops speltoides, BB) Y2001중균위다고패(SPL17-A1、SPL17-A2화SPL17-A3；SPL17-B1、SPL17-B2화SPL17-B3),재D기인조공체충조산양초(Aegilops tauschii, DD) Ae38중부검측도일충서렬(SPL17-D)； SPL17-A2여SPL17-B2, SPL17-A3여 SPL17-B3、SPL17-D량량지간서렬적일치성정도균대우99%,차여보통소맥(중국춘、형관35화쌍봉수)적 TaSPL17서렬구유교고적일치성,제시타문가능래원우공동적조선기인,병차재진화과정중고도보수。파기인 TaSPL17중적 tae-miR156식별위점비상보수,재근거단주수수화기인형다양성도선적 SubP1화SubP2군체중균미검측도tae-miR156식별위점존재변이감기。
Squamosa-promoter binding protein (SBP)-box genes, encoding plant-specific transcription factors, play an important role in plant development. Some members of the SBP-box gene family are regulated by miR156. In this study, we cloned the tae-MIR156 precursor gene, which could form a stem loop after its transcription. Sequence analysis showed that TaSPL3 and TaSPL17 had putative targets of tae-miR156 among the ten wheat SBP-box genes. The diploid donor species of hexaploid common wheat (Triticum aestivum, genome AABBDD), i.e., Triticum urartu (AA) UR209 and Aegilops spel-toides Y2001 (SS, closely related to BB) possessed more than one copy of SPL17 (SPL17-A1, SPL17-A2 and SPL17-A3 from Triticum urartu;SPL17-B1, SPL17-B2 and SPL17-B3 from Aegilops speltoides), while Aegilops tauschii (DD) Ae38 only possessed one (SPL17-D). The identities between nucleotide sequences of SPL17-A2 and SPL17-B2, SPL17-A3 and SPL17-B3 or SPL17-D were higher than 99%. They were highly similar with the sequence of TaSPL17 in common wheat cultivars Chinese Spring, Hengguan 35 and Shuangfengshou. These genes might originate from a common ancestor and were highly conserved in the process of evolution. The target site of tae-miR156 in TaSPL17 was also highly conserved in two subgroups consisted of accessions with diverse spike number per plant and genetic background.