环球中医药
環毬中醫藥
배구중의약
GLOBAL TCM
2014年
6期
420-426
,共7页
周新惠%龙丽莉%李春梅%赵荣华%张荣平%毛晓健
週新惠%龍麗莉%李春梅%趙榮華%張榮平%毛曉健
주신혜%룡려리%리춘매%조영화%장영평%모효건
生三七%蒸制熟三七%作用差异%补血%止血%活血
生三七%蒸製熟三七%作用差異%補血%止血%活血
생삼칠%증제숙삼칠%작용차이%보혈%지혈%활혈
Raw panax notoginseng%Steamed panax notoginseng%Activity differences%Tonifying blood%Stopping bleeding%Activating blood
目的：比较生三七、蒸制熟三七在补血、止血和活血方面的药理作用差异。方法(1)人工造成小鼠急性失血性贫血，给予生三七、蒸制熟三七，测定血常规指标及重要脏器(胸腺、脾脏、肾上腺)的重量，评价生三七、蒸制熟三七的补血作用。(2)用环磷酰胺造成小鼠血虚模型，给予生三七、蒸制熟三七，测定血常规指标及重要脏器(胸腺、脾脏、肾上腺)的重量，评价生三七、蒸制熟三七的补虚作用。(3)测定小鼠出血时间和凝血时间，评价生三七、蒸制熟三七的止血作用。(4)用静脉注射6%高分子右旋糖酐加冰水刺激造成小鼠微循环障碍，给予生三七、蒸制熟三七，观察小鼠耳廓微循环情况和全血切变率，评价生三七、蒸制熟三七对微循环和血液流变学的影响。结果(1)生三七、蒸制熟三七均能极显著升高失血性贫血小鼠红细胞总数、血红蛋白含量、白细胞总数，熟三七作用略好于生三七，但无显著性差异；对失血性贫血小鼠脏器重量的影响不显著。(2)生三七、蒸制熟三七对环磷酰胺所致血虚小鼠红细胞总数影响不显著，熟三七高剂量组能显著增加血虚小鼠血红蛋白含量，各给药组显著增加血虚小鼠白细胞总数(尤以复方阿胶浆组和熟三七高剂量组作用更显著)；与模型组比较，熟三七高、低剂量组各脏器指数均极显著升高，生三七高、低剂量组有升高的趋势，但作用不显著。(3)各给药组均能显著缩短小鼠出血时间；生三七能显著缩短小鼠凝血时间，熟三七则对凝血时间有缩短趋势，但影响不显著。(4)生三七、蒸制熟三七对微循环障碍小鼠耳廓微动脉口径影响不显著，对微静脉口径10分钟、20分钟时影响不显著，生三七高、低剂量组能显著增加微循环障碍小鼠30分钟时微静脉口径、毛细血管开放量和20分钟时毛细血管开放量；复方丹参片组可显著降低微循环障碍小鼠的全血黏度，熟三七高剂量组有降低的趋势，但作用不显著。结论生三七、蒸制熟三七对失血性贫血小鼠均有显著的补血作用，熟三七作用略好于生三七，但生、熟三七比较差异无显著性；熟三七高剂量组对环磷酰胺所致血虚小鼠作用显著，生三七高、低剂量组作用不显著，表明在提高免疫、补气作用方面熟三七好于生三七；在止血作用方面，生三七好于熟三七；在改善微循环方面，生三七好于熟三七，熟三七组仅高剂量组有一定作用，而且起效较慢；在改善血液黏度方面，熟三七好于生三七。
目的：比較生三七、蒸製熟三七在補血、止血和活血方麵的藥理作用差異。方法(1)人工造成小鼠急性失血性貧血，給予生三七、蒸製熟三七，測定血常規指標及重要髒器(胸腺、脾髒、腎上腺)的重量，評價生三七、蒸製熟三七的補血作用。(2)用環燐酰胺造成小鼠血虛模型，給予生三七、蒸製熟三七，測定血常規指標及重要髒器(胸腺、脾髒、腎上腺)的重量，評價生三七、蒸製熟三七的補虛作用。(3)測定小鼠齣血時間和凝血時間，評價生三七、蒸製熟三七的止血作用。(4)用靜脈註射6%高分子右鏇糖酐加冰水刺激造成小鼠微循環障礙，給予生三七、蒸製熟三七，觀察小鼠耳廓微循環情況和全血切變率，評價生三七、蒸製熟三七對微循環和血液流變學的影響。結果(1)生三七、蒸製熟三七均能極顯著升高失血性貧血小鼠紅細胞總數、血紅蛋白含量、白細胞總數，熟三七作用略好于生三七，但無顯著性差異；對失血性貧血小鼠髒器重量的影響不顯著。(2)生三七、蒸製熟三七對環燐酰胺所緻血虛小鼠紅細胞總數影響不顯著，熟三七高劑量組能顯著增加血虛小鼠血紅蛋白含量，各給藥組顯著增加血虛小鼠白細胞總數(尤以複方阿膠漿組和熟三七高劑量組作用更顯著)；與模型組比較，熟三七高、低劑量組各髒器指數均極顯著升高，生三七高、低劑量組有升高的趨勢，但作用不顯著。(3)各給藥組均能顯著縮短小鼠齣血時間；生三七能顯著縮短小鼠凝血時間，熟三七則對凝血時間有縮短趨勢，但影響不顯著。(4)生三七、蒸製熟三七對微循環障礙小鼠耳廓微動脈口徑影響不顯著，對微靜脈口徑10分鐘、20分鐘時影響不顯著，生三七高、低劑量組能顯著增加微循環障礙小鼠30分鐘時微靜脈口徑、毛細血管開放量和20分鐘時毛細血管開放量；複方丹參片組可顯著降低微循環障礙小鼠的全血黏度，熟三七高劑量組有降低的趨勢，但作用不顯著。結論生三七、蒸製熟三七對失血性貧血小鼠均有顯著的補血作用，熟三七作用略好于生三七，但生、熟三七比較差異無顯著性；熟三七高劑量組對環燐酰胺所緻血虛小鼠作用顯著，生三七高、低劑量組作用不顯著，錶明在提高免疫、補氣作用方麵熟三七好于生三七；在止血作用方麵，生三七好于熟三七；在改善微循環方麵，生三七好于熟三七，熟三七組僅高劑量組有一定作用，而且起效較慢；在改善血液黏度方麵，熟三七好于生三七。
목적：비교생삼칠、증제숙삼칠재보혈、지혈화활혈방면적약리작용차이。방법(1)인공조성소서급성실혈성빈혈，급여생삼칠、증제숙삼칠，측정혈상규지표급중요장기(흉선、비장、신상선)적중량，평개생삼칠、증제숙삼칠적보혈작용。(2)용배린선알조성소서혈허모형，급여생삼칠、증제숙삼칠，측정혈상규지표급중요장기(흉선、비장、신상선)적중량，평개생삼칠、증제숙삼칠적보허작용。(3)측정소서출혈시간화응혈시간，평개생삼칠、증제숙삼칠적지혈작용。(4)용정맥주사6%고분자우선당항가빙수자격조성소서미순배장애，급여생삼칠、증제숙삼칠，관찰소서이곽미순배정황화전혈절변솔，평개생삼칠、증제숙삼칠대미순배화혈액류변학적영향。결과(1)생삼칠、증제숙삼칠균능겁현저승고실혈성빈혈소서홍세포총수、혈홍단백함량、백세포총수，숙삼칠작용략호우생삼칠，단무현저성차이；대실혈성빈혈소서장기중량적영향불현저。(2)생삼칠、증제숙삼칠대배린선알소치혈허소서홍세포총수영향불현저，숙삼칠고제량조능현저증가혈허소서혈홍단백함량，각급약조현저증가혈허소서백세포총수(우이복방아효장조화숙삼칠고제량조작용경현저)；여모형조비교，숙삼칠고、저제량조각장기지수균겁현저승고，생삼칠고、저제량조유승고적추세，단작용불현저。(3)각급약조균능현저축단소서출혈시간；생삼칠능현저축단소서응혈시간，숙삼칠칙대응혈시간유축단추세，단영향불현저。(4)생삼칠、증제숙삼칠대미순배장애소서이곽미동맥구경영향불현저，대미정맥구경10분종、20분종시영향불현저，생삼칠고、저제량조능현저증가미순배장애소서30분종시미정맥구경、모세혈관개방량화20분종시모세혈관개방량；복방단삼편조가현저강저미순배장애소서적전혈점도，숙삼칠고제량조유강저적추세，단작용불현저。결론생삼칠、증제숙삼칠대실혈성빈혈소서균유현저적보혈작용，숙삼칠작용략호우생삼칠，단생、숙삼칠비교차이무현저성；숙삼칠고제량조대배린선알소치혈허소서작용현저，생삼칠고、저제량조작용불현저，표명재제고면역、보기작용방면숙삼칠호우생삼칠；재지혈작용방면，생삼칠호우숙삼칠；재개선미순배방면，생삼칠호우숙삼칠，숙삼칠조부고제량조유일정작용，이차기효교만；재개선혈액점도방면，숙삼칠호우생삼칠。
Objective To compare the pharmacological differences between raw and steamed pa-nax notoginseng on tonifying blood, stopping bleeding, and activating blood. Methods (1) The experi-ment was performed on mice artificially induced acute hemorrhagic anemia, which were administrated with raw or steamed panax notoginseng. The blood routine indexes were tested to evaluate the promoting effects of raw and steamed panax notoginseng on blood loss anemia mices. (2) The animal model was made by in-jecting cyclophosphamide intraperitoneally to induce mice blood deficiency. Raw and steamed panax notog-inseng were given orally for some time. The blood routine indexes and immune organs ( thymus,、spleen,、adrenal gland) weights were determined to observe the tonifying blood effects of raw and steamed panax no-toginseng on blood deficiency mice induced by cyclo-phosphamide. (3) The experimental mice were orally given raw or steamed panax notoginseng. The bleeding time and coagulation time in mice were determined to evaluate the stop-bleeding effects of raw and steamed panax notoginseng. (4) 6 % high molecular dex-tran ( iv) and ice water were used to cause mice microcirculation disturbance. Raw and steamed panax no-toginseng were given orally to mice for certain time. Microcirculation was observed and the blood shear rate was measured by automatic blood cell analyzer to evaluate the effects of raw and processed panax notogin-seng on microcirculation and hemorheology. Results (1) Raw and steamed panax notoginseng could both significantly increase RBC number, Hb content and WBC number of blood loss anemia mice. Steamed groups did slightly better, but there were no significant differences between raw and steamed. No significant influence was performed on immune organ weights of blood loss anemia mice. (2) Raw and steamed panax notoginseng both had no significant influence on RBC number of blood deficiency mice induced by cyclo-phosphamide. Compared with the model group, high dose group of steamed panax notoginseng could signifi-cantly increase the Hb content of blood deficiency mice. All medication administration teams significantly increased the WBC number in blood deficiency mice, especially compound E-Jiao slurry group and high dose of steamed panax notoginseng group. The immune organs ( thymus, spleen, adrenal gland) indexes of high and low dose steamed panax notoginseng group were quite significantly higher than model group. That of the high and low dose of raw panax notoginseng increased, but the effects were not significant. (3) Med-ication administration teams all could significantly short blooding time. Raw panax notoginseng could signif-icantly short coagulation time when the steamed ones had the tendency to reduce the blood coagulation time but not significant. (4) Raw and steamed panax notoginseng had no significant effect on artery width and also on vein width at 10min, 20min after administration. High and low dose of raw panax notoginseng both could increase vein width and the number of dilatation of capillary at 30min and the number of dilatation of capillary at 20 min. Conclusion Raw and steamed panax notoginseng both could significantly tonify blood for blood loss anemia mice and steamed sample did slightly better, but there were no significant differences. High dose group of steamed panax notoginseng could significantly increase the WBC number and thymus, spleen, adrenal gland indexes of blood deficiency mice cyclophosphamide induced. That of the high and low dose of raw panax notoginseng increased, but not significant. From this experiment,it can be seen that steamed panax notoginseng performs better on improving immunity and tonifying qi;raw panax notoginseng has better stop-bleeding;and on improving microcirculation effects, raw panax notoginseng is superior to steamed samples, of which high dose group has certain effects but effects slow;on promoting blood viscosi-ty, steamed panax notoginseng is better than the raw one.
