环球中医药
環毬中醫藥
배구중의약
GLOBAL TCM
2014年
6期
415-419
,共5页
李胜杰%李志同%杨伟宁%赵雅芳%解秸萍
李勝傑%李誌同%楊偉寧%趙雅芳%解秸萍
리성걸%리지동%양위저%조아방%해갈평
电针%上巨虚%尺泽%溃疡性结肠炎%肿瘤坏死因子α
電針%上巨虛%呎澤%潰瘍性結腸炎%腫瘤壞死因子α
전침%상거허%척택%궤양성결장염%종류배사인자α
Electroacupuncture%Shangjuxu (ST 37)%Chize (LU 5)%Ulcerative Colitis%Tumor necrosis factor α
目的：观察电针上巨虚穴、尺泽穴对溃疡性结肠炎大鼠不同器官中肿瘤坏死因子α( tumor necrosis factor α, TNF-α)含量及结肠黏膜病理改变的影响,探讨大肠下合穴上巨虚及肺经合穴尺泽对溃疡性结肠炎的影响及机制。方法将Wistar雄性大鼠28只随机分为正常组、模型组、上巨虚组及尺泽组,每组7只。采用乙酸灌肠法制备溃疡性结肠炎模型。于造模后第3天起,上巨虚组电针双侧上巨虚穴,尺泽组电针双侧尺泽穴,每次15分,每天一次,频率2/100 Hz,连续治疗7天；其余两组只抓取、束缚,不做治疗,每次15分,每天一次,7天后取材。放射免疫法测定肺、结肠TNF-α的含量；结肠组织HE染色,观察病理形态学变化。结果(1) TNF-α的含量：结肠组织中,模型组显著高于正常组(P<0．05),上巨虚组、尺泽组显著低于模型组(P值均<0．05),两治疗组比较未见显著性差异(P>0．05)；肺脏中,尺泽组TNF-α含量显著低于上巨虚组(P<0．05),其他组间则未见显著性差异(P值均>0．05)。(2)结肠与肺的比值,上巨虚组显著低于模型组和尺泽组(P值均<0．05),其他各组间未见显著性差异(P>0．05)。(3)模型组大鼠结肠光镜观察可见溃疡形成和炎症,上巨虚组和尺泽组大鼠结肠黏膜病理损伤减轻,其中上巨虚组减轻更为明显。结论(1)上巨虚和尺泽分别作为肺经的合穴和大肠的下合穴,均可降低TNF-α在结肠中的含量,有效阻抑UC所致的结肠炎症反应及改善结肠粘膜的病理改变,为“合主逆气而泄”、“合治内府”理论提供了实验依据。(2)上巨虚与尺泽作用的特异性靶器官可能不同,上巨虚对肠源性腹泻更有效,而尺泽对于肠炎引起的肺部损伤较上巨虚更有优势。
目的：觀察電針上巨虛穴、呎澤穴對潰瘍性結腸炎大鼠不同器官中腫瘤壞死因子α( tumor necrosis factor α, TNF-α)含量及結腸黏膜病理改變的影響,探討大腸下閤穴上巨虛及肺經閤穴呎澤對潰瘍性結腸炎的影響及機製。方法將Wistar雄性大鼠28隻隨機分為正常組、模型組、上巨虛組及呎澤組,每組7隻。採用乙痠灌腸法製備潰瘍性結腸炎模型。于造模後第3天起,上巨虛組電針雙側上巨虛穴,呎澤組電針雙側呎澤穴,每次15分,每天一次,頻率2/100 Hz,連續治療7天；其餘兩組隻抓取、束縳,不做治療,每次15分,每天一次,7天後取材。放射免疫法測定肺、結腸TNF-α的含量；結腸組織HE染色,觀察病理形態學變化。結果(1) TNF-α的含量：結腸組織中,模型組顯著高于正常組(P<0．05),上巨虛組、呎澤組顯著低于模型組(P值均<0．05),兩治療組比較未見顯著性差異(P>0．05)；肺髒中,呎澤組TNF-α含量顯著低于上巨虛組(P<0．05),其他組間則未見顯著性差異(P值均>0．05)。(2)結腸與肺的比值,上巨虛組顯著低于模型組和呎澤組(P值均<0．05),其他各組間未見顯著性差異(P>0．05)。(3)模型組大鼠結腸光鏡觀察可見潰瘍形成和炎癥,上巨虛組和呎澤組大鼠結腸黏膜病理損傷減輕,其中上巨虛組減輕更為明顯。結論(1)上巨虛和呎澤分彆作為肺經的閤穴和大腸的下閤穴,均可降低TNF-α在結腸中的含量,有效阻抑UC所緻的結腸炎癥反應及改善結腸粘膜的病理改變,為“閤主逆氣而洩”、“閤治內府”理論提供瞭實驗依據。(2)上巨虛與呎澤作用的特異性靶器官可能不同,上巨虛對腸源性腹瀉更有效,而呎澤對于腸炎引起的肺部損傷較上巨虛更有優勢。
목적：관찰전침상거허혈、척택혈대궤양성결장염대서불동기관중종류배사인자α( tumor necrosis factor α, TNF-α)함량급결장점막병리개변적영향,탐토대장하합혈상거허급폐경합혈척택대궤양성결장염적영향급궤제。방법장Wistar웅성대서28지수궤분위정상조、모형조、상거허조급척택조,매조7지。채용을산관장법제비궤양성결장염모형。우조모후제3천기,상거허조전침쌍측상거허혈,척택조전침쌍측척택혈,매차15분,매천일차,빈솔2/100 Hz,련속치료7천；기여량조지조취、속박,불주치료,매차15분,매천일차,7천후취재。방사면역법측정폐、결장TNF-α적함량；결장조직HE염색,관찰병리형태학변화。결과(1) TNF-α적함량：결장조직중,모형조현저고우정상조(P<0．05),상거허조、척택조현저저우모형조(P치균<0．05),량치료조비교미견현저성차이(P>0．05)；폐장중,척택조TNF-α함량현저저우상거허조(P<0．05),기타조간칙미견현저성차이(P치균>0．05)。(2)결장여폐적비치,상거허조현저저우모형조화척택조(P치균<0．05),기타각조간미견현저성차이(P>0．05)。(3)모형조대서결장광경관찰가견궤양형성화염증,상거허조화척택조대서결장점막병리손상감경,기중상거허조감경경위명현。결론(1)상거허화척택분별작위폐경적합혈화대장적하합혈,균가강저TNF-α재결장중적함량,유효조억UC소치적결장염증반응급개선결장점막적병리개변,위“합주역기이설”、“합치내부”이론제공료실험의거。(2)상거허여척택작용적특이성파기관가능불동,상거허대장원성복사경유효,이척택대우장염인기적폐부손상교상거허경유우세。
Objective To probe into the mechanisms of electroacupuncture ( EA) at Shangjuxu (ST 37, lower He-sea acupoint) and Chize (LU 5, He-sea acupoint) for treatment of the ulcerative colitis ( UC) , through observing TNF-Alpha content in different organs and morphology of colonic mucosa in UC rats. Methods Twenty-eight male Wistar rats were randomly divided into 4 groups: a control group, a model group, an EA-Shangjuxu ( EA-ST 37 ) group and an EA-Chize ( EA-LU 5 ) group, 7 rats in each group. The UC rat model was made with by enema with 10% acetic acid, and the change of TNF-αcontent in the Lung and Colon and morphology of colonic mucosa after EA at Shangjuxu ( ST 37 ) and Chize ( LU 5) were observed. The treatment began from day 3 after establishing models. EA(2/100HZ) was applied to bilateral Chize ( LU 5 ) and Chize ( LU 5 ) for 15 min, once daily for 7 days. The control group and model group bound only for 15min, once daily for 7 days. TNF-αcontent in the lung and colon was deter-mined by RIA. Morphology of colonic mucosa were observed under a light microscope by hematoxylin-eosin (HE) Staining. Results (1) EA at Shangjuxu (ST 37) and Chize (LU 5) could significantly decrease the TNF-α level in the colic tissues with significant differences as compared with the model group (P<0. 05). Compared with the control group, the TNF-α content was obviously increased in the model group (P<0. 05). No significant difference can be seen between EA-Shangjuxu group and EA-Chize group. The TNF-α content in EA-Chize group was significantly decreased in the lung tissues comparing with EA-Shangjuxu group(P<0. 05). No significant difference can be seen among the other groups(P>0. 05). . (2) The ratio of TNF levels in the colon and lung in EA-Shangjuxu group was significantly decreased com-paring with the model group (P<0. 05) and EA-Chize group(P<0. 05). No significant differences among the other groups(P >0. 05). (3) Ulceration and inflammation of colon can be observed in the model group. Pathological lesions of colonic mucosa were reduced in the EA-Shangjuxu group and EA-Chize group. The EA-Shangjuxu group was more obvious. Conclusions ( 1 ) EA at Shangjuxu ( ST 37 ) and Chize (LU 5) could significantly decrease the TNF-αlevel in the colic tissues, effectively prevent inflam-matory response of colon and improving pathological changes of colonic mucosa, which has provided the ex-perimental basis for the theory of"he-sea points governing vomiting and diarrhea" and"Curing viscera dis-eases by he-sea points". (2) Maybe Shangjuxu (ST 37) and Chize (LU 5) have different target organs. Shangjuxu ( ST 37 ) is better for enterogenic diarrhea, while Chize ( LU 5 ) has more advantages than Shangjuxu (ST 37) for lung injury caused by enteritis.
