环球中医药
環毬中醫藥
배구중의약
GLOBAL TCM
2014年
6期
401-405
,共5页
刘文兰%油红捷%车念聪%白辰%唐佐青
劉文蘭%油紅捷%車唸聰%白辰%唐佐青
류문란%유홍첩%차념총%백신%당좌청
肝纤维化%骨髓间充质干细胞%肝损伤%一贯煎%肝肾阴虚
肝纖維化%骨髓間充質榦細胞%肝損傷%一貫煎%肝腎陰虛
간섬유화%골수간충질간세포%간손상%일관전%간신음허
Liver fibrosis%Bone marrow mesenchymal stem cells%Hepatic injury%Yiguan-jian%Yin deficiency of liver and kidney
目的：该研究观察骨髓间充质干细胞对肝纤维化的逆转作用,了解一贯煎对该过程的作用,阐释一贯煎逆转肝纤维化的机制。方法60只雄性Sprague-Dawlay大鼠随机分为5组,即正常对照组、模型组、阳性对照组、骨髓间充质干细胞组、一贯煎加骨髓间充质干细胞组,每组12只。除正常对照组外,均建立肝纤维化恢复模型,分离、培养和标记骨髓间充质干细胞,在CCl4停止注射后3天,骨髓间充质干细胞组每只动物经尾静脉注射5×106 mmol/L浓度1 mol骨髓间充质干细胞；一贯煎加骨髓间充质干细胞组每只大鼠尾静脉注射等量的骨髓间充质干细胞外,给予一贯煎浓缩液2 ml/200 g灌胃,每天1次。阳性对照组给予秋水仙碱灌胃,0．25 mg/kg,每天1次。正常对照组和模型组按相同方法灌服等量生理盐水。阳性对照组、正常对照组和模型组经尾静脉注射等量生理盐水。4周后,检测各组大鼠血清谷丙转氨酶、谷草转氨酶等肝功能指标；通过HE染色评价肝脏炎症活动度(G)和纤维化程度(S)；荧光显微镜下示踪肝组织中骨髓间充质干细胞。结果(1)骨髓间充质干细胞组和一贯煎加骨髓间充质干细胞组较模型组和阳性组在改善肝功能、减轻炎症、逆转肝纤维化的作用较好,P<0．05。一贯煎加骨髓间充质干细胞组较骨髓间充质干细胞组在改善肝功能、逆转纤维化的作用更明显,P<0．05。(2)骨髓间充质干细胞组和一贯煎加骨髓间充质干细胞组骨髓间充质干细胞在肝脏分布面积较模型组增大,P<0．05；一贯煎加骨髓间充质干细胞组较其他组差异显著,P<0．05。结论骨髓间充质干细胞具有逆转肝纤维化的作用,一贯煎对该过程具有促进作用；一贯煎能够促进骨髓间充质干细胞募集到肝脏,从而发挥逆转肝纤维化的作用。
目的：該研究觀察骨髓間充質榦細胞對肝纖維化的逆轉作用,瞭解一貫煎對該過程的作用,闡釋一貫煎逆轉肝纖維化的機製。方法60隻雄性Sprague-Dawlay大鼠隨機分為5組,即正常對照組、模型組、暘性對照組、骨髓間充質榦細胞組、一貫煎加骨髓間充質榦細胞組,每組12隻。除正常對照組外,均建立肝纖維化恢複模型,分離、培養和標記骨髓間充質榦細胞,在CCl4停止註射後3天,骨髓間充質榦細胞組每隻動物經尾靜脈註射5×106 mmol/L濃度1 mol骨髓間充質榦細胞；一貫煎加骨髓間充質榦細胞組每隻大鼠尾靜脈註射等量的骨髓間充質榦細胞外,給予一貫煎濃縮液2 ml/200 g灌胃,每天1次。暘性對照組給予鞦水仙堿灌胃,0．25 mg/kg,每天1次。正常對照組和模型組按相同方法灌服等量生理鹽水。暘性對照組、正常對照組和模型組經尾靜脈註射等量生理鹽水。4週後,檢測各組大鼠血清穀丙轉氨酶、穀草轉氨酶等肝功能指標；通過HE染色評價肝髒炎癥活動度(G)和纖維化程度(S)；熒光顯微鏡下示蹤肝組織中骨髓間充質榦細胞。結果(1)骨髓間充質榦細胞組和一貫煎加骨髓間充質榦細胞組較模型組和暘性組在改善肝功能、減輕炎癥、逆轉肝纖維化的作用較好,P<0．05。一貫煎加骨髓間充質榦細胞組較骨髓間充質榦細胞組在改善肝功能、逆轉纖維化的作用更明顯,P<0．05。(2)骨髓間充質榦細胞組和一貫煎加骨髓間充質榦細胞組骨髓間充質榦細胞在肝髒分佈麵積較模型組增大,P<0．05；一貫煎加骨髓間充質榦細胞組較其他組差異顯著,P<0．05。結論骨髓間充質榦細胞具有逆轉肝纖維化的作用,一貫煎對該過程具有促進作用；一貫煎能夠促進骨髓間充質榦細胞募集到肝髒,從而髮揮逆轉肝纖維化的作用。
목적：해연구관찰골수간충질간세포대간섬유화적역전작용,료해일관전대해과정적작용,천석일관전역전간섬유화적궤제。방법60지웅성Sprague-Dawlay대서수궤분위5조,즉정상대조조、모형조、양성대조조、골수간충질간세포조、일관전가골수간충질간세포조,매조12지。제정상대조조외,균건립간섬유화회복모형,분리、배양화표기골수간충질간세포,재CCl4정지주사후3천,골수간충질간세포조매지동물경미정맥주사5×106 mmol/L농도1 mol골수간충질간세포；일관전가골수간충질간세포조매지대서미정맥주사등량적골수간충질간세포외,급여일관전농축액2 ml/200 g관위,매천1차。양성대조조급여추수선감관위,0．25 mg/kg,매천1차。정상대조조화모형조안상동방법관복등량생리염수。양성대조조、정상대조조화모형조경미정맥주사등량생리염수。4주후,검측각조대서혈청곡병전안매、곡초전안매등간공능지표；통과HE염색평개간장염증활동도(G)화섬유화정도(S)；형광현미경하시종간조직중골수간충질간세포。결과(1)골수간충질간세포조화일관전가골수간충질간세포조교모형조화양성조재개선간공능、감경염증、역전간섬유화적작용교호,P<0．05。일관전가골수간충질간세포조교골수간충질간세포조재개선간공능、역전섬유화적작용경명현,P<0．05。(2)골수간충질간세포조화일관전가골수간충질간세포조골수간충질간세포재간장분포면적교모형조증대,P<0．05；일관전가골수간충질간세포조교기타조차이현저,P<0．05。결론골수간충질간세포구유역전간섬유화적작용,일관전대해과정구유촉진작용；일관전능구촉진골수간충질간세포모집도간장,종이발휘역전간섬유화적작용。
Objective To investigate the effect of bone marrow mesenchymal stem cells on liver fi-brosis reversal, understand the role of Yiguanjian on this process, and interpret the mechanism of Yiguan-jian on the recovery of liver fibrosis. Methods we established liver fibrosis rat model. 60 male SD rats were divided into 5 groups:Normal group, model group, western medicine group, mesenchymal stem cells group and Yiguanjian combined with bone marrow mesenchymal stem cells group, There were 12 rats in each group. Except the normal group, hepatic fibrosis model was established. Isolation, culture and la-beled bone marrow mesenchymal stem cells. On the third day after CCl4 injection, each rat in mesenchy-mal stem cells group was given intravenous injection of 5 × 106 mmol/L 1 mol bone marrow mesenchymal stem cells. Except equal amounts of bone marrow mesenchymal stem cells, each rat in Yiguanjian com-bined with bone marrow mesenchymal stem cells group was given Yiguanjian(2 ml/200 g d)by intragastric administration. Western medicine group was given colchicine (0. 25 mg/kg d) by intragastric administra-tion. The normal group and model group were fed with same volume of saline daily. Western medicine group, normal group and model group was given intravenous injection of same volume of saline daily. After 4 weeks,the serum ALT and AST were tested, the HE staining were used to evaluate the degree of liver in-flammation and fibrosis ( G) ( S) , Bone marrow mesenchymal stem cells in liver tissue were observed under fluorescence microscop. Results Compared with the model group and western medicine group,mesenchymal stem cells group and Yiguanjian combined with bone marrow mesenchymal stem cells group had better effect in the improvement of liver function, reduce inflammation, reversing hepatic fibrosis, P < 0. 05. Compared with the mesenchymal stem cells group , Yiguanjian combined with bone marrow mesenchymal stem cells group had better effect on improving the liver function, reversing hepatic fibrosis, P < 0. 05. Compared with the model group, the area of bone marrow mesenchymal stem cells in liver was greater in mesenchymal stem cells group and Yiguanjian combined with bone marrow mesenchymal stem cells group, P < 0. 05. Compared with the other groups, the area of bone marrow mesenchymal stem cells in liver was greater in Yiguanjian combined with bone marrow mesenchymal stem cells group, P< 0. 05. Conclusion Bone marrow mesen-chymal stem cells can reverse liver fibrosis , Yiguanjian can promote this process, Yiguanjian can promote bone marrow mesenchymal stem cell recruitment to the liver, thus play an effect of recovery of liver fibrosis.
