CAJ | 학술논문

为了构建食用油过氧化值快速检测分析方法，以食用油为试验材料，利用可见分光光度计测定碘与淀粉的显色变化，建立食用油过氧化值的检测模型，探讨测定波长、饱和碘化钾添加量、反应时间、淀粉指示剂添加量、溶剂混合比例和油脂种类等因素对测定结果的影响。通过模型建立和验证、盲样验证及精密度分析，结果表明：检测条件对测定结果影响均不显著；当取样质量为（0.100±0.010） g，入射光波长为535nm时，食用油过氧化值模型为： y=28.856x+0.1331（y为过氧化值， mmol/kg， x为溶液吸光度）， R2=0.9985。模型预测值与国标法测定值高度线性相关，盲样验证相对误差均＜10%，检出限为0.79mmol/kg，符合国家标准的相关要求，基于可见分光光度法的食用油过氧化值检测方法是可行的。
위료구건식용유과양화치쾌속검측분석방법，이식용유위시험재료，이용가견분광광도계측정전여정분적현색변화，건립식용유과양화치적검측모형，탐토측정파장、포화전화갑첨가량、반응시간、정분지시제첨가량、용제혼합비례화유지충류등인소대측정결과적영향。통과모형건립화험증、맹양험증급정밀도분석，결과표명：검측조건대측정결과영향균불현저；당취양질량위（0.100±0.010） g，입사광파장위535nm시，식용유과양화치모형위： y=28.856x+0.1331（y위과양화치， mmol/kg， x위용액흡광도）， R2=0.9985。모형예측치여국표법측정치고도선성상관，맹양험증상대오차균＜10%，검출한위0.79mmol/kg，부합국가표준적상관요구，기우가견분광광도법적식용유과양화치검측방법시가행적。
In order to develop rapid determination method of peroxide value in edible oils, using edible oils as raw materials, calibrations which based on chromomeric reaction of I2-KI solution were developed and validated for de-termination of peroxide value, and effects on determination of the varying condition, such as detective wavelength, amount of saturated potassium iodide, reaction temperature, amount of starch indicator, types of oil samples and ratio of organic solvent were explored. Method was validated by Chinese national standard methods. Results showed that experi-mental conditions had no significant effect on determination. When edible oil sample was (0.100±0.010) g, calibration was y=28.856x+0.1331 (where x is the value of the absorbance, y is peroxide value), R2=0.9985. Linear correlativity of determination of peroxide value between two methods was very well, relative deviation of blind samples experiment′s data were less than 10%, according with national standard requirement, the lowest detectable limitation was 0.79mmol/kg. Proposed method was workable.