CAJ | 학술논문

目的：探讨山楂叶总黄酮对脑缺血大鼠脑组织PI3K/Akt信号通路的影响。方法雄性SD大鼠随机分为假手术组、模型组、银杏叶片组和山楂叶总黄酮组，每组20只。后3组采用双侧颈总动脉结扎法制备慢性脑缺血大鼠模型，银杏叶片组给予银杏叶片5.2 mg/kg，山楂叶总黄酮组给予山楂叶总黄酮140 mg/kg灌胃，模型组和假手术组给予等量的生理盐水。免疫组化法检测PI3K、Akt蛋白的表达，干湿重法检测脑组织水含量，甲酰胺法测定血脑屏障通透性。结果与模型组比较，银杏叶片组和山楂叶总黄酮组PI3K、Akt蛋白均明显升高（ P＜0.05，P＜0.01），脑组织含水量及伊文思蓝含量显著降低（P＜0.05，P＜0.01）。结论山楂叶总黄酮对缺血性脑损伤具有保护作用，其作用机制可能与调控PI3 K/Akt信号通路有关。
목적：탐토산사협총황동대뇌결혈대서뇌조직PI3K/Akt신호통로적영향。방법웅성SD대서수궤분위가수술조、모형조、은행협편조화산사협총황동조，매조20지。후3조채용쌍측경총동맥결찰법제비만성뇌결혈대서모형，은행협편조급여은행협편5.2 mg/kg，산사협총황동조급여산사협총황동140 mg/kg관위，모형조화가수술조급여등량적생리염수。면역조화법검측PI3K、Akt단백적표체，간습중법검측뇌조직수함량，갑선알법측정혈뇌병장통투성。결과여모형조비교，은행협편조화산사협총황동조PI3K、Akt단백균명현승고（ P＜0.05，P＜0.01），뇌조직함수량급이문사람함량현저강저（P＜0.05，P＜0.01）。결론산사협총황동대결혈성뇌손상구유보호작용，기작용궤제가능여조공PI3 K/Akt신호통로유관。
Objective To study the effects of Hawthorn leaves flavonoids ( HLF) on the PI3K/Akt signaling pathway in rats with cerebral ischemia .Methods Chronic cerebral ischemia rat model was constructed by bilateral carot-id artery ligation.PI3K and Akt protein expression was assessed by immunohistochemical assay .Water content of brain tissue was detected by dry-wet method , while brain barrier permeability was measured by formamide method blood .Re-sults Compared with the model group , significant up-regulations in PI3K and Akt proteins were observed in Ginkgo bi-loba group and HLF group (P<0.05).Significant reductions in cerebral water content and EB content were also observed (P<0.05).Conclusion HLF can protect the brain from ischemia injury , probably via regulation of PI3K/Akt signaling pathway .