CAJ | 학술논문

目的：通过观察灯盏花素对对比剂诱导的急性肾损伤大鼠肾脏Na＋，K＋-ATP酶活性的影响，探讨灯盏花素对对比剂肾损伤大鼠早期的保护作用机制。方法采用健康成年SD大鼠及糖尿病大鼠模型，设正常对照组（ NS组）、对比剂对照组（ CM组）、灯盏花素治疗组（ Bre组）及糖尿病对比剂对照组（ DCM组）、糖尿病灯盏花素治疗组（ DBre）。 NS组予10 mL/kg生理盐水尾静脉注射，其余各组予等量低渗对比剂。 NS和CM组再予20 mg/（ kg· d）腹腔注射、Bre组予等量灯盏花素。一次性腹腔注射链脲佐菌素60 mg/kg建立糖尿病模型，成模糖尿病大鼠注射低渗对比剂后分别予生理盐水及灯盏花素腹腔注射。免疫组化SABC法检测Na＋，K＋-ATP酶在大鼠肾脏的定位和表达，观察Na＋，K＋-ATP酶活性变化。结果灯盏花素可使注射对比剂后受抑制的Na＋，K＋-ATP酶活性表达明显上调（P＜0.05），这种作用在糖尿病模型组大鼠（DCM组与DBre组）表现更明显（P＜0.01）。结论灯盏花素对大鼠对比剂急性肾损伤有一定的保护作用，该作用可能与减轻对比剂对Na＋，K＋-ATP酶活性的抑制作用有关。
목적：통과관찰등잔화소대대비제유도적급성신손상대서신장Na＋，K＋-ATP매활성적영향，탐토등잔화소대대비제신손상대서조기적보호작용궤제。방법채용건강성년SD대서급당뇨병대서모형，설정상대조조（ NS조）、대비제대조조（ CM조）、등잔화소치료조（ Bre조）급당뇨병대비제대조조（ DCM조）、당뇨병등잔화소치료조（ DBre）。 NS조여10 mL/kg생리염수미정맥주사，기여각조여등량저삼대비제。 NS화CM조재여20 mg/（ kg· d）복강주사、Bre조여등량등잔화소。일차성복강주사련뇨좌균소60 mg/kg건립당뇨병모형，성모당뇨병대서주사저삼대비제후분별여생리염수급등잔화소복강주사。면역조화SABC법검측Na＋，K＋-ATP매재대서신장적정위화표체，관찰Na＋，K＋-ATP매활성변화。결과등잔화소가사주사대비제후수억제적Na＋，K＋-ATP매활성표체명현상조（P＜0.05），저충작용재당뇨병모형조대서（DCM조여DBre조）표현경명현（P＜0.01）。결론등잔화소대대서대비제급성신손상유일정적보호작용，해작용가능여감경대비제대Na＋，K＋-ATP매활성적억제작용유관。
Objective To study the effects of Breviscapine on the Na +, K+-ATPase activity in kidney tissues of rats with contrast-induced acute kidney injury .Methods SD rats and diabetic rat models were randomly divided into five groups:normal control group (Group NS), contrast media control group (Group CM), Bre treatment group (Group Bre), diabetic model with contrast media group (Group DCM), and diabetic model with Bre treatment group (Group DBre) .Localization and activity of Na +, K+-ATPase in kidney tissues was detected by immunohistochemistry staining method.Results The activity of Na +, K+-ATPase in kidney tissues, which was inhibited by contrast media , was sig-nificantly enhanced with breviscapine ( P<0.05) , and it was significantly more prominent in diabetic rats ( Group DCM and DBre) (P<0.01).Conclusion Breviscapine provides a certain protection on contrast -induced acute kidney injury in rats, probably via lightening the inhibition of Na +, K+-ATPase activity caused by contrast media .