现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2014年
10期
282-282,284
,共2页
高效液相%豆芽%2,4-D%测定
高效液相%豆芽%2,4-D%測定
고효액상%두아%2,4-D%측정
HPLC%bean sprouts%2,4-D%determination
使用高效液相色谱法检测豆芽中2，4-D残留量，用氢氧化钠溶液作为提取剂，采用超声波法提取样品中的2，4-D，经SPE净化，采用紫外检测器检测；用保留时间进行定性分析，外标法进行定量计算。结果表明：标准曲线为Y=4024.1873x-113.9999，相关系数0.9999，线性范围为0.05~10.00μg/mL，方法检出限为0.02 mg/kg，回收率为90.3%～104.7%，相对标准偏差为0.52%～11.18%。
使用高效液相色譜法檢測豆芽中2，4-D殘留量，用氫氧化鈉溶液作為提取劑，採用超聲波法提取樣品中的2，4-D，經SPE淨化，採用紫外檢測器檢測；用保留時間進行定性分析，外標法進行定量計算。結果錶明：標準麯線為Y=4024.1873x-113.9999，相關繫數0.9999，線性範圍為0.05~10.00μg/mL，方法檢齣限為0.02 mg/kg，迴收率為90.3%～104.7%，相對標準偏差為0.52%～11.18%。
사용고효액상색보법검측두아중2，4-D잔류량，용경양화납용액작위제취제，채용초성파법제취양품중적2，4-D，경SPE정화，채용자외검측기검측；용보류시간진행정성분석，외표법진행정량계산。결과표명：표준곡선위Y=4024.1873x-113.9999，상관계수0.9999，선성범위위0.05~10.00μg/mL，방법검출한위0.02 mg/kg，회수솔위90.3%～104.7%，상대표준편차위0.52%～11.18%。
Sodium hydrate was used as extracting agent,the ultrasonicwave method was used to extract 2,4-D in bean sprouts.The samples were purified by SPE and detected by UV detector.RT was used in qualitative analysis,and external standard method was used in quantitative calculation. The results showed that the standard curve was Y=4 024.187 3x-113.999 9,the correlation coefficient was 0.999 9,the linearity range was 0.05~10.00μg/mL,the detection limit of method was 0.02 mg/kg,the standard recovery was 90.3%~104.7%,and RSD was 0.52%~11.18%.
