现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2014年
10期
77-78,84
,共3页
巴戟天%毛状根诱导%发根农杆菌
巴戟天%毛狀根誘導%髮根農桿菌
파극천%모상근유도%발근농간균
Morinda officinalis How%hairy roots inducement%Agrobacterium rhizogene
为探索巴戟天毛状根的诱导条件，该研究选择不同发根农杆菌ATCC15834、AR10、Msu440菌株诱导巴戟天无菌苗的幼芽、叶片、叶柄、茎段等外植体形成毛状根，并研究了共培养时间和乙酰丁香酮(AS)浓度等因素对巴戟天毛状根转化率的影响。结果表明：巴戟天毛状根的最佳诱导条件为：以幼芽为外植体，ATCC15834为转化菌，侵染时间20 min，并在培养基中添加300μmol/L乙酰丁香酮条件下，巴戟天毛状根的诱导转化率最高，可达36.7%。
為探索巴戟天毛狀根的誘導條件，該研究選擇不同髮根農桿菌ATCC15834、AR10、Msu440菌株誘導巴戟天無菌苗的幼芽、葉片、葉柄、莖段等外植體形成毛狀根，併研究瞭共培養時間和乙酰丁香酮(AS)濃度等因素對巴戟天毛狀根轉化率的影響。結果錶明：巴戟天毛狀根的最佳誘導條件為：以幼芽為外植體，ATCC15834為轉化菌，侵染時間20 min，併在培養基中添加300μmol/L乙酰丁香酮條件下，巴戟天毛狀根的誘導轉化率最高，可達36.7%。
위탐색파극천모상근적유도조건，해연구선택불동발근농간균ATCC15834、AR10、Msu440균주유도파극천무균묘적유아、협편、협병、경단등외식체형성모상근，병연구료공배양시간화을선정향동(AS)농도등인소대파극천모상근전화솔적영향。결과표명：파극천모상근적최가유도조건위：이유아위외식체，ATCC15834위전화균，침염시간20 min，병재배양기중첨가300μmol/L을선정향동조건하，파극천모상근적유도전화솔최고，가체36.7%。
To establish the inducement conditions for hairy roots of Morinda officinalis How,A grobacterium rhizogene strains of ATCC15834,AR10,Msu440 were selected to induce the transformation ration of explants of buds,leaf,leafstalk and stem of Morinda officinalis How aseptic seedling into hairy root.The effect of infection time and concentrations of acetosyringone(AS)on the transformation ratio were also studied in this paper.The results showed that the better conditions for inducing hairy roots was as followsyoung buds as explant,infected by strain ATCC15834 for 20 min and then cultured in MS with 300μmol/L acetosyringone,in this conditions,the inducting rate reached 36.7%.
