CAJ | 학술논문

目的：研究CXC趋化因子配体（CXCL）5受体（CXCR2）蛋白在前列腺癌细胞株PC-3、DU145和LNCaP细胞中的表达情况及CXCL5对LNCaP细胞生长的影响。方法应用Western印迹技术检测CXCR2在3种前列腺癌细胞株PC-3、DU145和 LNCaP细胞中的表达情况，筛选出表达量最高的细胞株，然后用MTT法检测5、10、20 ng/ml 浓度CXCL5对CXCR2蛋白表达量最高的前列腺癌细胞生长的影响。结果在PC-3、DU145和LNCaP细胞中均有CXCR2蛋白的表达，LNCaP细胞中最高（1.65±0.089），DU145细胞次之（1.44±0.107），PC-3细胞中最低（0.36±0.056）（P＜0.05）；5、10、20 ng/ml CXCL5浓度组作用 LNCaP细胞6 d后，吸光度值分别为0.55±0.06，0.61±0.08和0.73±0.10，与对照组（0.52±0.14）比较，20 ng/ml CXCL5浓度组增长率明显增高（ P＜0.05）。结论 CXCL5可通过作用于其自身受体CXCR2进而促进LNCaP细胞生长。
목적：연구CXC추화인자배체（CXCL）5수체（CXCR2）단백재전렬선암세포주PC-3、DU145화LNCaP세포중적표체정황급CXCL5대LNCaP세포생장적영향。방법응용Western인적기술검측CXCR2재3충전렬선암세포주PC-3、DU145화 LNCaP세포중적표체정황，사선출표체량최고적세포주，연후용MTT법검측5、10、20 ng/ml 농도CXCL5대CXCR2단백표체량최고적전렬선암세포생장적영향。결과재PC-3、DU145화LNCaP세포중균유CXCR2단백적표체，LNCaP세포중최고（1.65±0.089），DU145세포차지（1.44±0.107），PC-3세포중최저（0.36±0.056）（P＜0.05）；5、10、20 ng/ml CXCL5농도조작용 LNCaP세포6 d후，흡광도치분별위0.55±0.06，0.61±0.08화0.73±0.10，여대조조（0.52±0.14）비교，20 ng/ml CXCL5농도조증장솔명현증고（ P＜0.05）。결론 CXCL5가통과작용우기자신수체CXCR2진이촉진LNCaP세포생장。
Objective To study the expression of the receptor CXCR 2 of CXCL5 in prostate cancer cell lines PC-3, DU145, LNCaP and the effect of CXCL5 to the growth of LNCaP prostate cancer cell line .Methods The expression of CXCR2 in three prostate cancer lines PC-3, DU145 and LNCaP were measured by Western blot .The highest expression cell line was screened , and the effect of 5, 10 and 20 ng/ml CXCL5 on the growth of the highest expression cell line was detected by MTT .Results The proteins of CXCR2 were expressed in PC-3, DU145 and LNCaP cell lines.The expressions of CXCR2 from high-level to low-level were LNCaP cell line(1.65±0.089), DU145 cell line(1.44±0.107)and PC-3 cell line (0.36±0.056)(P<0.05);After 5,10 and 20 ng/ml CXCL5 acted on LNCaP cell line for 6 days, the absorbance values were 0.55 ±0.06 ,0.61 ±0.08 and 0.73 ±0.10 , respectively .Compared with that in control group , 20 ng/ml CXCL5 significantly increased growth rate of LNCaP cell line .Conclusions CXCL5 can promote growth of LNCaP cell line by acting on autoreceptor .