CAJ | 학술논문

目的：探讨人纤维介素（fgl2）对心肌微血管内皮细胞（MMVECs）增殖、迁移的影响。方法制作并包装fgl2的RNA干扰慢病毒，转染MMVECs。实验分为单纯MMVECs（对照组）、空载质粒慢病毒（ GFP组）、fgl2短发夹 RNA（ shRNA）慢病毒（ fgl2-RNAi-LV组）。稳定转染4 d后，实时定量RT-PCR（ qRT-PCR）检测各组fgl2 mRNA表达，MTT检测细胞增殖；Transwell 检测细胞迁移能力。结果 qRT-PCR 结果显示，与对照组与GFP组比较，fgl2-RNAi-LV组的fgl2表达明显下调，MMVECs的增殖能力与迁移能力增强（P＜0.05），而对照组与 GFP 组无明显差异。结论转染fgl2shRNA慢病毒载体后MMVECs的fgl2表达水平明显下调， fgl2shRNA慢病毒转染MMVECs，MMVECs的增殖能力与迁移能力增强。
목적：탐토인섬유개소（fgl2）대심기미혈관내피세포（MMVECs）증식、천이적영향。방법제작병포장fgl2적RNA간우만병독，전염MMVECs。실험분위단순MMVECs（대조조）、공재질립만병독（ GFP조）、fgl2단발협 RNA（ shRNA）만병독（ fgl2-RNAi-LV조）。은정전염4 d후，실시정량RT-PCR（ qRT-PCR）검측각조fgl2 mRNA표체，MTT검측세포증식；Transwell 검측세포천이능력。결과 qRT-PCR 결과현시，여대조조여GFP조비교，fgl2-RNAi-LV조적fgl2표체명현하조，MMVECs적증식능력여천이능력증강（P＜0.05），이대조조여 GFP 조무명현차이。결론전염fgl2shRNA만병독재체후MMVECs적fgl2표체수평명현하조， fgl2shRNA만병독전염MMVECs，MMVECs적증식능력여천이능력증강。
Objective To construct RNAi lentivirus vector of fgl 2 gene and explore the effect of fgl 2 gene on microvascular endothe-lial cells ( MMVECs) of myocardial proliferation and migration.Methods RNAi lentivirus vector of fgl 2 gene was constructed and infected into MMVECs.Cells were divided into pure MMVECs ( control group ) , empty plasmid lentivirus ( GFP group ) , fgl2 short hairpin RNA (shRNA group), lentivirus (fgl2-RNAi-LV group).4 days later transfected into cells, quantitative real-time RT-PCR (Qrt-PCR) was used to detect fgl2 mRNA expression , MTT was used to detect cell proliferation , Transwell was used to detect cell migration.Results Compared with control and GFP groups , fgl2 expression was down-regulated and proliferation and migration of MMVECs were increased in fgl 2-RNAi-LV group ( P<0.05 ).There were no significant differences in above indexes between control and GFP groups .Conclusions fgl2 expression in MMVECs transfected with fgl 2-RNAi-LV is down-regulated and proliferation and migration of MMVECs are increased.