CAJ | 학술논문

目的：观察血管内皮生长因子（ VEGF-D）在非小细胞肺癌（ NSCLC）组织中央部位、侵犯边缘和癌周肺组织的表达情况及肺癌组织微淋巴管密度（ LMVD）与淋巴结转移、淋巴管侵犯和淋巴管生成的相关性。方法选择人 NSCLC 新鲜组织样本96例，应用免疫组织化学法和实时适量（QRT）-PCR法检测NSCLC组织中央部位、侵犯边缘和癌周肺组织的VEGF-D蛋白及mRNA表达情况，应用单克隆抗体（D2-40）标记淋巴管，检测NSCLC组织中的LWVD。结果 QRT-PCR显示，VEGF-D mRNA在肿瘤边缘部位的表达明显高于在肿瘤中央部位的表达，而且VEGF-D mRNA的表达亦高于其在周围肺组织的表达。免疫组织结果显示了同样的表达不均一性。肿瘤边缘VEGF-D高表达组淋巴结转移的发生率明显高于低表达组，而且VEGF-D高表达组其淋巴管侵犯的发生率亦明显增高。肿瘤边缘高LWVD 组的 VEGF-D mRNA的表达也明显高于低密度组。结论肿瘤侵犯边缘的VEGF-D的高表达与淋巴结转移和淋巴管侵犯密切相关，参与了NSCLC的淋巴管生成和淋巴结转移过程。
목적：관찰혈관내피생장인자（ VEGF-D）재비소세포폐암（ NSCLC）조직중앙부위、침범변연화암주폐조직적표체정황급폐암조직미림파관밀도（ LMVD）여림파결전이、림파관침범화림파관생성적상관성。방법선택인 NSCLC 신선조직양본96례，응용면역조직화학법화실시괄량（QRT）-PCR법검측NSCLC조직중앙부위、침범변연화암주폐조직적VEGF-D단백급mRNA표체정황，응용단극륭항체（D2-40）표기림파관，검측NSCLC조직중적LWVD。결과 QRT-PCR현시，VEGF-D mRNA재종류변연부위적표체명현고우재종류중앙부위적표체，이차VEGF-D mRNA적표체역고우기재주위폐조직적표체。면역조직결과현시료동양적표체불균일성。종류변연VEGF-D고표체조림파결전이적발생솔명현고우저표체조，이차VEGF-D고표체조기림파관침범적발생솔역명현증고。종류변연고LWVD 조적 VEGF-D mRNA적표체야명현고우저밀도조。결론종류침범변연적VEGF-D적고표체여림파결전이화림파관침범밀절상관，삼여료NSCLC적림파관생성화림파결전이과정。
Objective To detect the expression of VEGF-D in non-small cell lung carcinoma (NSCLC)tissue and to evaluate the re-lationship between the expression of VEGF-D and lymphangiogenesis and lymph node metastasis .Methods The expressions of VEGF-D protein and mRNA in NSCLC tumors were detected by immunohistochemistry and quantitative real -time polymerase chain reaction ( QRT-PCR) .Results In marginal region ,the expression of VEGF-D mRNA was significantly higher than that in tumor center , and the expression of VEGF-D was also higher than that in peritumoral lung tissue .The group with high expression of VEGF-D in marginal region had a higher incidence of lymph node metastasis compared with the group with low expression .Furthermore, the group with high expression of VEGF-D in marginal region had a higher incidence of lymphatic invasion .The group with high peritumoral lymphatic vessel density ( LVD) had higher expression of VEGF-D mRNA compared with the group with low peritumoral LVD .Conclusions The expression of VEGF-D at invasive edge is significantly associated with lymph node metastasis or lymphatic invasion in patients with NSCLC , and may be involved in regulation of lymphangiogenesis and lymph node metastasis in NSCLC .