CAJ | 학술논문

目的：探讨戴安娜全自动血型仪在血型鉴定及交叉配血中的应用。方法使用全自动血型仪和传统试管法检测2300份标本的血型；用微柱凝胶法和聚凝胺法对900例患者进行交叉配血试验。结果全自动血型仪和试管法检测血型一次性判读准确率分别为99．87％、100．00％；在900例交叉配血试验中，微柱凝胶法配血不合30例，主侧不合3例，次侧不合27例，将微柱凝胶法配血不合的标本用聚凝胺法检测，主侧不合3例，次侧不合3例。结论全自动血型仪用于 ABO 血型和 RhD 血型检测安全、快速、可靠、灵敏度高；微柱凝胶法进行交叉配血较敏感，但假凝集及不规则抗体的存在会导致配血困难，耗时较长；聚凝胺法简便、快速、假阳性较少，但有可能导致抗体漏检。
목적：탐토대안나전자동혈형의재혈형감정급교차배혈중적응용。방법사용전자동혈형의화전통시관법검측2300빈표본적혈형；용미주응효법화취응알법대900례환자진행교차배혈시험。결과전자동혈형의화시관법검측혈형일차성판독준학솔분별위99．87％、100．00％；재900례교차배혈시험중，미주응효법배혈불합30례，주측불합3례，차측불합27례，장미주응효법배혈불합적표본용취응알법검측，주측불합3례，차측불합3례。결론전자동혈형의용우 ABO 혈형화 RhD 혈형검측안전、쾌속、가고、령민도고；미주응효법진행교차배혈교민감，단가응집급불규칙항체적존재회도치배혈곤난，모시교장；취응알법간편、쾌속、가양성교소，단유가능도치항체루검。
Objective To evaluate the accuracy and reliability of Diana automated blood grouping analyzer in blood grouping and cross matching.Methods 2 300 patients′ABO and RhD blood groups were examined by conventional tube test and the fully auto-mated blood grouping analyzer and 900 patients′samples were tested using Diana automated blood grouping for blood cross matc-hing,and it was compared with polymatching method.Results The analyzer′s accuracy rate of blood grouping by two methods were 99.87% and 100.00%.The incompatibility occurred in 30 specimens in automatic blood type instrument,in 3 specimens in manual polymatching method.Conclusion The results of Diana automated blood grouping analyzer used for blood grouping and cross matc-hing blood testing are reliable.Its experimental operation is normalized and standardized with an advantage of low incidence of human er-ror.Moreover,the experimental results can be permanently preserved,which provides a convenience to search for medical proof.