CAJ | 학술논문

目的：了解新生儿 ABO 正反定型符合率，研究提高新生儿血型测定准确性的方法。方法用传统试管法检测当地新生儿 ABO 血型，以微柱凝胶卡和改良试管法、增强剂法及常规试管法、纸片法、微板法、聚凝胺法等7种方法检测抗-A、抗-B、抗-D 抗体的效价，并进行新生儿血型检测比较。结果新生儿 ABO 正反定型符合率为83％（913/1100）；检测15份抗-A、抗-B、抗-D 抗体的效价，敏感性最高是改良试管法和增强剂法，最差的是纸片法，新生儿标本检测结果类似。结论ABO 正反定型应增加血浆（血清）量并延长孵育时间或选用增强剂，从而提高新生儿血型检测的准确性。
목적：료해신생인 ABO 정반정형부합솔，연구제고신생인혈형측정준학성적방법。방법용전통시관법검측당지신생인 ABO 혈형，이미주응효잡화개량시관법、증강제법급상규시관법、지편법、미판법、취응알법등7충방법검측항-A、항-B、항-D 항체적효개，병진행신생인혈형검측비교。결과신생인 ABO 정반정형부합솔위83％（913/1100）；검측15빈항-A、항-B、항-D 항체적효개，민감성최고시개량시관법화증강제법，최차적시지편법，신생인표본검측결과유사。결론ABO 정반정형응증가혈장（혈청）량병연장부육시간혹선용증강제，종이제고신생인혈형검측적준학성。
Objective To understand the neonatal ABO type positive and reverse typing consistent rate and to improve the accu-racy of the methods of neonatal blood.Methods The traditional tube method was used to detect the local neonatal ABO blood.The method of micro gel cards,improved tube method,enhancer and traditional tube method,paper,micro-plate,polybrene method to de-tect anti A,anti B,anti D titer,and neonatal blood detection comparator.Results Neonatal ABO positive and negative stereo types compliance rate was 83% (913/1 100);Detecting 15 copies of anti A,B,D titer,the highest sensitivity was improved intensifier tube method and the worst is the method of paper.The method of neonatal specimens was with the similar results.Conclusion ABO positive and negative stereo types should be increased by plasma (serum)levels and extending the incubation time,or using the"en-hancer"of this study,thereby improving the accuracy of the newborn blood testing.