CAJ | 학술논문

目的：对基于量子点荧光的免疫渗滤快速定量检测血清 C 反应蛋白（CRP）方法进行初步探索，旨在建立一种较好的快速定量检测方法。方法采用双抗夹心法原理在免疫渗滤板上建立自制量子点和量子点-抗体复合物快速免疫检测法，其结果在紫外光照射下进行荧光定性检测。采用激光器和荧光光谱仪相结合的方法，可对荧光检测结果进行定量分析。结果定性检测到 CRP 的最低浓度为0．156 mg/L；定量检测 CRP 浓度范围在0．1～100．0 mg/L 的样本，其检测荧光值与浓度有线性对应关系，线性拟合方程为：log（Y ）＝0．563log（X）＋4．570，r2＝0．958。结论荧光免疫渗透快速定量法可对血清 CRP 进行定量检测；量子点免疫标记技术平台具有开发新型免疫诊断试剂的潜力。
목적：대기우양자점형광적면역삼려쾌속정량검측혈청 C 반응단백（CRP）방법진행초보탐색，지재건립일충교호적쾌속정량검측방법。방법채용쌍항협심법원리재면역삼려판상건립자제양자점화양자점-항체복합물쾌속면역검측법，기결과재자외광조사하진행형광정성검측。채용격광기화형광광보의상결합적방법，가대형광검측결과진행정량분석。결과정성검측도 CRP 적최저농도위0．156 mg/L；정량검측 CRP 농도범위재0．1～100．0 mg/L 적양본，기검측형광치여농도유선성대응관계，선성의합방정위：log（Y ）＝0．563log（X）＋4．570，r2＝0．958。결론형광면역삼투쾌속정량법가대혈청 CRP 진행정량검측；양자점면역표기기술평태구유개발신형면역진단시제적잠력。
Objective To study the feasibility of using fluorescent immunofiltration test based on quantum dots (QDs)for rapid and quantitative detection of C-reactive protein.Methods Based on homemade QDs and QDs-antibody bioconjugates,an immune de-tection method was established via the double antibodies sandwich technique on the immunochromatography card.The test results could be read under the irradiation of UV light,and quantitative results could be measured through the combination of a laser and fluorescent spectroscopy.Results Under UV light irradiation,the minimum detection concentration of CRP was 0.156 mg/L.U-sing the quantitative detection method,the fluorescent intensities on the cards could be established a linear relationship with the con-centration of CRP,and the linear equation was that log(Y )=0.563 log(X)+4.570,r2 =0.958.Conclusion The fluorescent quan-tum dot immunofiltration assay can be used for quantitative detection of CRP;The quantum dots immuno-labels have the potential to develop new type of immune-diagnostic reagents.