CAJ | 학술논문

目的：以离子色谱法为基础，建立血清总锂离子测定的候选参考方法并评价其性能。方法采用一种简单的样本前处理方法，用新鲜患者血清样本、IFCC-RELA 样本等对方法的精密度进行评估；用加标回收实验评价方法的准确性。结果锂离子标准曲线线性方程为：Y ＝0．8171X -0．0013，r2＝0．99995，方法的检出限为6μg/L；测量血清样本的不精密度（CV）小于1．0％；样本的加标回收率在99％～101％之间。结论成功建立了血清锂候选参考方法，可用于血清锂离子项目的量值溯源和标准化，为血清锂常规检测系统向参考方法/参考物质溯源提供了有效途径。
목적：이리자색보법위기출，건립혈청총리리자측정적후선삼고방법병평개기성능。방법채용일충간단적양본전처리방법，용신선환자혈청양본、IFCC-RELA 양본등대방법적정밀도진행평고；용가표회수실험평개방법적준학성。결과리리자표준곡선선성방정위：Y ＝0．8171X -0．0013，r2＝0．99995，방법적검출한위6μg/L；측량혈청양본적불정밀도（CV）소우1．0％；양본적가표회수솔재99％～101％지간。결론성공건립료혈청리후선삼고방법，가용우혈청리리자항목적량치소원화표준화，위혈청리상규검측계통향삼고방법/삼고물질소원제공료유효도경。
Objective To establish a candidate reference method for the determination of serum lithium based on ion chromatog-raphy and evaluate its analytical performance.Methods A simple sample treatment procedure,which can be remove the proteins and/or organics in human serum,has been developed for the determination of serum lithium.Method precision was evaluated with different concentration of fresh human serum and EQA sample RELA-A/B.Method accuracy was investigated with the recovery ex-periments in fresh human serum and RELA-A/B sample.Results The linear equation was Y =0.817 1X -0.001 3 with a correla-tion coefficient of 0.999 95 under the optimum experimental conditions,the detection limit (3S/N)for lithium was 6 μg/L and the imprecision was less than 1.0%.The results of the recovery experiments indicated that the recoveries were reasonable for the deter-mination of serum lithium,in a range of 99%-101%.Conclusion The candidate reference method of lithium was successfully es-tablished and which can be used for traceability and standardization.It may provide an effective way for routine testing of lithium traceable to the reference method/reference material.