CAJ | 학술논문

目的：探讨初发过敏性紫癜（HSP）患儿细胞免疫、体液免疫功能变化及其临床意义。方法采用流式细胞术检测156例 HSP 患儿外周血 T 淋巴细胞 CD3＋、CD3＋ CD4＋、CD3＋ CD8＋、CD3＋ CD4＋ CD8＋，B 淋巴细胞 CD3- CD19＋及 NK 细胞CD3- CD16＋ CD56＋的表达水平，采用散射免疫比浊法检测血清免疫球蛋白 IgG、IgA、IgM，以及补体 C3、C4的水平，采用免疫化学发光法检测 IgE 水平，并与25例健康儿童进行比较。结果HSP 患儿 CD3＋、CD3＋ CD4＋百分比及 CD4＋/CD8＋比值均显著下降，CD3- CD19＋百分比显著上升，与健康儿童相比差异均有统计学意义（P ＜0．01），CD3＋ CD4＋ CD8＋百分比上升（P ＜0．05）， CD16＋ CD56＋百分比下降（P ＜0．05）。HSP 患儿的 IgA、IgE、IgG、C3水平升高，与健康儿童比较差异有统计学意义（P ＜0．05）。结论HSP 患儿存在细胞免疫及体液免疫水平失调造成的免疫功能紊乱，淋巴细胞亚群、免疫球蛋白及补体的检测对于从细胞免疫及体液免疫角度了解患儿的免疫功能，探讨疾病的发病机制、辅助诊断及预后有重要意义。
목적：탐토초발과민성자전（HSP）환인세포면역、체액면역공능변화급기림상의의。방법채용류식세포술검측156례 HSP 환인외주혈 T 림파세포 CD3＋、CD3＋ CD4＋、CD3＋ CD8＋、CD3＋ CD4＋ CD8＋，B 림파세포 CD3- CD19＋급 NK 세포CD3- CD16＋ CD56＋적표체수평，채용산사면역비탁법검측혈청면역구단백 IgG、IgA、IgM，이급보체 C3、C4적수평，채용면역화학발광법검측 IgE 수평，병여25례건강인동진행비교。결과HSP 환인 CD3＋、CD3＋ CD4＋백분비급 CD4＋/CD8＋비치균현저하강，CD3- CD19＋백분비현저상승，여건강인동상비차이균유통계학의의（P ＜0．01），CD3＋ CD4＋ CD8＋백분비상승（P ＜0．05）， CD16＋ CD56＋백분비하강（P ＜0．05）。HSP 환인적 IgA、IgE、IgG、C3수평승고，여건강인동비교차이유통계학의의（P ＜0．05）。결론HSP 환인존재세포면역급체액면역수평실조조성적면역공능문란，림파세포아군、면역구단백급보체적검측대우종세포면역급체액면역각도료해환인적면역공능，탐토질병적발병궤제、보조진단급예후유중요의의。
Objective To discuss the change of cellular immune and humoral immune function in children patients with Henoch-Schonlein purpura(HSP)and to investigate its clinical significance.Methods The expression levels of T lymphocyte CD3 + ,CD3 +CD4 + ,CD3 + CD8 + ,CD3 + CD4 + CD8 + ,B lymphocyte CD3 - CD19 + and NK lymphocyte CD3 - CD16 + CD56 + in periphetal blood of 156 patients with HSP was detected by flow cytometry.The level of immunioglobins(IgG,IgA,IgM)and complement(C3,C4)of serum were evaluated by nephelometry immunoassay.The level of immunioglobin IgE was measured by immunochemiluminometric assays and compared with 25 healthy controls.Results Compared with control group,the ratio of CD4 +/CD8 + and the contents of CD3 + ,CD4 + cells in HSP patients were decreased significantly(P <0.01),while the content of CD19 + was significantly increased (P <0.01).The content of CD16 + CD56 + was decreased(P <0.05 ),while the content of CD3 + CD4 + CD8 + was increased(P <0.05),the content of CD8 + had no obvious change(P >0.01).The level of IgA and IgE were increased significantly(P <0.01).The level of IgG and C3 were increased (P <0.05),The level of IgM and C4 had no obvious change(P >0.05).Conclusion The cellular immune and humoral immune function in children with HSP are in disorder.To detect lymphocyte subsets,immunioglobin and com-plement will be of important significance to understand the immune function from the perspective of the cellular immune and humor-al immune to explore the pathogenic mechanisms and to assist diagnosis and patient′s prognosis.