CAJ | 학술논문

目的：探讨支原体、衣原体和抗精子抗体的检测对男性不育症的应用价值。方法采用培养法、免疫层析法、ELISA法，对102例不育男性和42例已育男性进行支原体、衣原体和抗精子抗体的检测及精液常规的分析。根据结果分为单纯支原体或（和）衣原体阳性组71例，单纯抗精子抗体阳性组21例，支原体或（和）衣原体和抗精子抗体阳性组8例，支原体、衣原体和抗精子抗体阴性组44例，对4组的精液常规主要指标进行比较。结果不育男性支原体、衣原体、抗精子抗体阳性率明显高于已育男性（P ＜0．05）；单纯支原体或（和）衣原体阳性组，单纯抗精子抗体阳性组及支原体或（和）衣原体和抗精子抗体阳性组的精子密度、活动率、活力明显低于阴性组，精子的畸形率、液化时间、白细胞数明显高于阴性组，差异有统计学意义（P ＜0．05）。结论支原体、衣原体的感染及抗精子抗体的产生对精液多项指标有明显影响，精液质量下降，从而引起男性不育症的发生。
목적：탐토지원체、의원체화항정자항체적검측대남성불육증적응용개치。방법채용배양법、면역층석법、ELISA법，대102례불육남성화42례이육남성진행지원체、의원체화항정자항체적검측급정액상규적분석。근거결과분위단순지원체혹（화）의원체양성조71례，단순항정자항체양성조21례，지원체혹（화）의원체화항정자항체양성조8례，지원체、의원체화항정자항체음성조44례，대4조적정액상규주요지표진행비교。결과불육남성지원체、의원체、항정자항체양성솔명현고우이육남성（P ＜0．05）；단순지원체혹（화）의원체양성조，단순항정자항체양성조급지원체혹（화）의원체화항정자항체양성조적정자밀도、활동솔、활력명현저우음성조，정자적기형솔、액화시간、백세포수명현고우음성조，차이유통계학의의（P ＜0．05）。결론지원체、의원체적감염급항정자항체적산생대정액다항지표유명현영향，정액질량하강，종이인기남성불육증적발생。
Objective To explore the application value of detection of Mycoplasma,Chlamydia and anti-sperm antibodies for male infertility.Methods Culture method,immune chromatography,and enzyme linked immunosorbent assay were adopted for the detection of Mycoplasma,Chlamydia,and anti-sperm antibodies respectively in 102 cases of infertile males and 42 cases fertile males.And the routine semen analysis was proceed as well.All the subjects were divided into 4 groups according to the detection re-sults:simple Mycoplasma or/and Chlamydia positive group(71 cases),simple anti-sperm antibodies positive group(21 cases),My-coplasma and Chlamydia or/and anti-sperm antibodies positive group(8 cases),Mycoplasma,Chlamydia and anti-sperm antibodies negative group(44 cases).The main indexes of semen routine were compared among 4 groups.Results The positive rates of Myco-plasma,Chlamydia and anti-sperm antibodies in infertile males were significantly higher than those of fertile males (P <0.05).The sperm densities,activity rates,activity of simple Mycoplasma or/and Chlamydia positive group,simple anti-sperm antibodies posi-tive group,and Mycoplasma and Chlamydia or /and anti-sperm antibodies positive group were significantly lower than the nega-tive group,while the sperm malformation rates,liquefaction times,white blood cell counts of the three groups were significantly higher than the negative group(P <0.05 ).Conclusion Mycoplasma,Chlamydia infection and anti-sperm antibodies production have significant effect on the indexes of semen,which cause decline in semen quality and the occurrence of male infertility.