CAJ | 학술논문

目的：探讨经纤支镜活检取材并进行原代肺癌细胞培养的方法，以提高培养成功率．方法30例经纤支镜活检肺癌组织进行原代细胞培养，对肿瘤组织形态与原代细胞培养成功率相关性进行分析．结果30例经纤支镜取材肺癌细胞培养病例，原代培养成功17例，占总例数的56.67%．纤支镜下呈菜花样生长肺癌组织培养成功率84.62%（11/13）；肿瘤沿管壁生长管腔狭窄表面有或无突起的培养成功率分别为66.67%（2/3）和37.5%（3/8）；球状肿块质硬培养成功率16.67%（1/6），菜花状与其他采用确切概率法比较，差异有统计学意义（＜0.05）．结论经纤支镜活检肺癌组织体外原代细胞培养简单易行，阳性率可达56.67%，且原代培养成功率与纤支镜下肿瘤组织形态相关．
목적：탐토경섬지경활검취재병진행원대폐암세포배양적방법，이제고배양성공솔．방법30례경섬지경활검폐암조직진행원대세포배양，대종류조직형태여원대세포배양성공솔상관성진행분석．결과30례경섬지경취재폐암세포배양병례，원대배양성공17례，점총례수적56.67%．섬지경하정채화양생장폐암조직배양성공솔84.62%（11/13）；종류연관벽생장관강협착표면유혹무돌기적배양성공솔분별위66.67%（2/3）화37.5%（3/8）；구상종괴질경배양성공솔16.67%（1/6），채화상여기타채용학절개솔법비교，차이유통계학의의（＜0.05）．결론경섬지경활검폐암조직체외원대세포배양간단역행，양성솔가체56.67%，차원대배양성공솔여섬지경하종류조직형태상관．
Objetive To investigate a method of collecting lung cancer cells with bronchofibroscopic biopsy for primary culture and to improve the success rate of primary culture. Methods Thirty lung cancer specimens were obtained through bronchoscopic biopsy for primary culture. The correlation of cancer morphology under bronchofi-broscopy and success rate of primary culture was analyzed. Results Among the lung cancer specimens obtained through bronchoscopic biopsy, primary culture was successful in 17 of 30 cases (56.67%) . The success rate of cauliflower-like tumor mass under bronchofibroscopy was 84.62% (11/13) . The success rate of infiltrating tumor mass under bronchial mucosa with luminal stenosis with or without cristate were 66.67% (2/3) and 37.5%(3/8), respectively. The primary culture of a globular and stiff tumor mass was successful only 1 in 6 cases (16.67%) .Conclusions The primary culture of lung cancer cells obtained from bronchofibroscopic biopsy is simple and effective with a total success rate of 56.67%. Furthermore, the success rate of primary culture is signifi-cantly correlated with the cancer morphology under bronchofibroscopy.