中国急救医学
中國急救醫學
중국급구의학
CHINESE JOURNAL OF CRITICAL CARE MEDICINE
2014年
4期
358-360
,共3页
肺纤维化%程序性死亡因子( PD-1 )%大鼠%硫化氢
肺纖維化%程序性死亡因子( PD-1 )%大鼠%硫化氫
폐섬유화%정서성사망인자( PD-1 )%대서%류화경
Pulmonary fibrosis%PD-1%Rats%Hydrogen sulfide
目的：研究硫化氢对肺纤维化大鼠的治疗作用及对肺组织中程序性死亡因子-1（ PD-1）表达的影响。方法36只SD大鼠，随机分为对照组、模型组和硫氢化钠干预组。模型组和干预组气管内注射博来霉素诱导肺纤维化，对照组在相同条件下给予生理盐水。第2天起干预组大鼠每天经尾静脉注射硫氢化钠（3．12 mg/kg），其余两组相同条件下给予生理盐水。治疗第7天和第28天，处死动物取出肺组织，进行病理学观察；用免疫组化和RT-PCR观察各组肺组织PD-1蛋白及mRNA表达的水平。结果病理学观察显示，与模型组比较，干预组肺泡炎和纤维化程度均减轻；与模型组比较，干预组PD-1蛋白及mRNA表达显著降低。结论硫化氢可能通过抑制PD-1的过度表达，对实验性大鼠肺纤维化具有良好的治疗作用。
目的：研究硫化氫對肺纖維化大鼠的治療作用及對肺組織中程序性死亡因子-1（ PD-1）錶達的影響。方法36隻SD大鼠，隨機分為對照組、模型組和硫氫化鈉榦預組。模型組和榦預組氣管內註射博來黴素誘導肺纖維化，對照組在相同條件下給予生理鹽水。第2天起榦預組大鼠每天經尾靜脈註射硫氫化鈉（3．12 mg/kg），其餘兩組相同條件下給予生理鹽水。治療第7天和第28天，處死動物取齣肺組織，進行病理學觀察；用免疫組化和RT-PCR觀察各組肺組織PD-1蛋白及mRNA錶達的水平。結果病理學觀察顯示，與模型組比較，榦預組肺泡炎和纖維化程度均減輕；與模型組比較，榦預組PD-1蛋白及mRNA錶達顯著降低。結論硫化氫可能通過抑製PD-1的過度錶達，對實驗性大鼠肺纖維化具有良好的治療作用。
목적：연구류화경대폐섬유화대서적치료작용급대폐조직중정서성사망인자-1（ PD-1）표체적영향。방법36지SD대서，수궤분위대조조、모형조화류경화납간예조。모형조화간예조기관내주사박래매소유도폐섬유화，대조조재상동조건하급여생리염수。제2천기간예조대서매천경미정맥주사류경화납（3．12 mg/kg），기여량조상동조건하급여생리염수。치료제7천화제28천，처사동물취출폐조직，진행병이학관찰；용면역조화화RT-PCR관찰각조폐조직PD-1단백급mRNA표체적수평。결과병이학관찰현시，여모형조비교，간예조폐포염화섬유화정도균감경；여모형조비교，간예조PD-1단백급mRNA표체현저강저。결론류화경가능통과억제PD-1적과도표체，대실험성대서폐섬유화구유량호적치료작용。
Objective To investigate the effect of hydrogen sulfide on PD -1 expression in the lung of rats with experimental pulmonary fibrosis .Methods Thirty six male SD rats were randomly divided into three groups:the control group , the pulmonary fibrosis group and the treatment group .The model of pulmonary fibrosis was produced by bleomycin endotracheally , while the control group was given saline under the same condition .From the second day the rats in the treatment group were treated with 3.12 mg/kg NaHS ( total volumn of 2 ml) ig, while the other two groups'rats were treated with 2 ml saline ig instead .On the seventh day and the twenty -eight day , the lungs were isolated .Pathological changes in the lungs were evaluated by HE stain and Masson's trichrome stain .The mRNA expression of PD-1 was evaluated by quantitative RT -PCR analysis , and the immunohistochemical method was used to investigate the change of PD -1 protein.Results The degree of alveolitis and pulmonary fibrosis in the treatment group was alleviated compared with that of the model group .The expression level of PD -1 and mRNA in the treatment group was significantly decreased compared with the model group ( P<0.05 ) .Conclusion Over-expression of PD-1 may be an important mechanism in the pathogenesis of pulmonary fibrosis .Hydrogen sulfide might play pivotal role in curing the experimental pulmonary fibrosis in SD rats by suppressing excessive expressing of lung PD -1.
