CAJ | 학술논문

目的：观察脑缺血-再灌注对血清NO和CO的影响及颅痛定的干预。方法随机抽样2011-01～2012-12收治的心肺复苏术后35例患者，随机分为治疗组20例，对照组15例。两组患者心肺复苏后立即给予一般性治疗，治疗组起病后即加用颅痛定60 mg，每日3次，持续3 d用药。于心肺复苏术后6、24、48、72 h抽血测定NO、CO浓度。硝酸还原酶法测定血清NO浓度，可见分光光度计法测定CO浓度。结果人脑缺血-再灌注6、24、48、72 h后外周静脉血中NO浓度增加，其中脑缺血＜5 min再灌注48 h达到高峰；人脑缺血-再灌注6 h后CO浓度最大，之后回落，其中脑缺血＜5 min再灌注24 h达到高峰；颅痛定降低脑缺血-再灌注6～24 h CO浓度，增加NO浓度，而在缺血-再灌注24～72 h降低NO浓度。结论颅痛定可能通过调控机体内NO和CO浓度，发挥抗脑缺血-再灌注损伤作用。
목적：관찰뇌결혈-재관주대혈청NO화CO적영향급로통정적간예。방법수궤추양2011-01～2012-12수치적심폐복소술후35례환자，수궤분위치료조20례，대조조15례。량조환자심폐복소후립즉급여일반성치료，치료조기병후즉가용로통정60 mg，매일3차，지속3 d용약。우심폐복소술후6、24、48、72 h추혈측정NO、CO농도。초산환원매법측정혈청NO농도，가견분광광도계법측정CO농도。결과인뇌결혈-재관주6、24、48、72 h후외주정맥혈중NO농도증가，기중뇌결혈＜5 min재관주48 h체도고봉；인뇌결혈-재관주6 h후CO농도최대，지후회락，기중뇌결혈＜5 min재관주24 h체도고봉；로통정강저뇌결혈-재관주6～24 h CO농도，증가NO농도，이재결혈-재관주24～72 h강저NO농도。결론로통정가능통과조공궤체내NO화CO농도，발휘항뇌결혈-재관주손상작용。
Objective To observe the effects of L -tetrahydropalmatine(L -THP)on nitric oxide (NO) and carbon monoxide (CO) concentration in plasma during cerebral ischemia reperfusion . Methods Cardiopulmonary resuscitation ( CPR) of 35 cases of postoperative patients were divided into 2 groups in random:treatment group(n=20)and control group(n=15).Two groups of patients after CPR immediately to give general treatment .Treatment group after the onset of the cranial pain .Nitrate reductive enzymatic method was used to detect the serum content of NO; visible spectrophotometer method was used to detect the serum content of CO .Results NO content was elevated at 6, 24, 48, 72 hour, and peaked at 48 hour when cerebral ischemia occurred within 5 minutes;CO content peaked at 6 hour, while peaked at 24 hour when cerebral ischemia occurred within 5 minutes;L-THP significantly increased NO concentrations and reduced CO concentrations after cerebral ischemia reperfusion from 6 to 24 hour, while reduced NO concentrations after cerebral ischemia reperfusion from 24 to 72 hour. Conclusion L-THP probably has a good effect on cerebral ischemia -reperfusion injury by regulating the NO and CO contents .