中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2013年
10期
1042-1050
,共9页
子宫体部平滑肌%收缩%分娩发动%蛋白质组学
子宮體部平滑肌%收縮%分娩髮動%蛋白質組學
자궁체부평활기%수축%분면발동%단백질조학
corpus myometrium%contraction%parturition%proteomics
目的：筛选与人子宫平滑肌收缩相关的蛋白质以探讨人子宫体部平滑肌收缩及分娩发动的分子机制。方法：应用双向凝胶电泳技术分离人足月自然临产和未临产子宫体部平滑肌组织总蛋白，经考马斯亮蓝染色、图像分析、识别差异表达的蛋白质点，基质辅助激光解吸电离飞行时间质谱得到相应的肽质量指纹图谱，搜索数据库鉴定部分差异表达蛋白质；Western印迹对3个差异表达蛋白进行验证。结果：建立了人足月自然临产和未临产子宫体部平滑肌的双向凝胶电泳图谱，质谱鉴定得到自然临产和未临产之间的表达差异大于2倍的蛋白质点20个，其功能涉及细胞骨架、钙结合力、分子伴侣、能量代谢、信号转导和抗氧化能力等； Western印迹验证结果与蛋白质组学结果一致。结论：筛选并鉴定了20个子宫体部平滑肌收缩相关蛋白质，提示细胞骨架改变、钙结合能力增强是分娩发动的重要原因。
目的：篩選與人子宮平滑肌收縮相關的蛋白質以探討人子宮體部平滑肌收縮及分娩髮動的分子機製。方法：應用雙嚮凝膠電泳技術分離人足月自然臨產和未臨產子宮體部平滑肌組織總蛋白，經攷馬斯亮藍染色、圖像分析、識彆差異錶達的蛋白質點，基質輔助激光解吸電離飛行時間質譜得到相應的肽質量指紋圖譜，搜索數據庫鑒定部分差異錶達蛋白質；Western印跡對3箇差異錶達蛋白進行驗證。結果：建立瞭人足月自然臨產和未臨產子宮體部平滑肌的雙嚮凝膠電泳圖譜，質譜鑒定得到自然臨產和未臨產之間的錶達差異大于2倍的蛋白質點20箇，其功能涉及細胞骨架、鈣結閤力、分子伴侶、能量代謝、信號轉導和抗氧化能力等； Western印跡驗證結果與蛋白質組學結果一緻。結論：篩選併鑒定瞭20箇子宮體部平滑肌收縮相關蛋白質，提示細胞骨架改變、鈣結閤能力增彊是分娩髮動的重要原因。
목적：사선여인자궁평활기수축상관적단백질이탐토인자궁체부평활기수축급분면발동적분자궤제。방법：응용쌍향응효전영기술분리인족월자연임산화미임산자궁체부평활기조직총단백，경고마사량람염색、도상분석、식별차이표체적단백질점，기질보조격광해흡전리비행시간질보득도상응적태질량지문도보，수색수거고감정부분차이표체단백질；Western인적대3개차이표체단백진행험증。결과：건립료인족월자연임산화미임산자궁체부평활기적쌍향응효전영도보，질보감정득도자연임산화미임산지간적표체차이대우2배적단백질점20개，기공능섭급세포골가、개결합력、분자반려、능량대사、신호전도화항양화능력등； Western인적험증결과여단백질조학결과일치。결론：사선병감정료20개자궁체부평활기수축상관단백질，제시세포골가개변、개결합능력증강시분면발동적중요원인。
Objective: To explore the molecular mechanism of human corpus myometrium contraction related proteins and parturition.
<br> Methods: The proteins of human corpus of myometrium tissues from full term non-in labor (38-41 weeks amenorrhea) and full term in labor (38-41weeks amenorrhea) gravidas were separated by 2-dimensional gel electrophoresis (2-GE), respectively. Then gels were stained by Coomassie brilliant blue G250, scanned by Image scanner and analyzed with PDQuest software. The differentially expressed protein spots of corpus myometrium between the 2 groups were identified by peptide mass finger print based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database searching. Three identified differentially expressed proteins were conifrmed by Western blot.
<br> Results: Well resolved and reproducible 2D-GE maps of human corpus myometrium from non-in labor and in-labor gravidas were acquired. Twenty more than 2-fold differentially expressed protein spots were identified by MALDI-TOF-MS. These proteins were involved in cell structure, calcium-binding, chaperone, energy metabolism, signal transduction, and antioxidant.
<br> Conclusion: Twenty contraction related proteins of human corpus myometrium have been identiifed, indicating that cell structure and calcium-binding are important reasons for the contraction of human corpus myometrium.
