CAJ | 학술논문

目的：：研究淫羊藿苷的抗抑郁及对皮质酮致PC12细胞损伤的保护作用。方法：采用大/小鼠强迫游泳、小鼠悬尾三种实验模型，将动物随机分为对照组、淫羊藿苷低剂量组、淫羊藿苷高剂量组、阿米替林组，观察药物对大/小鼠强迫游泳不动时间、小鼠悬尾不动时间的影响；并在细胞水平建立皮质酮损伤PC12细胞模型，观察淫羊藿苷的细胞保护作用。结果：在大/小鼠强迫游泳和小鼠悬尾实验中，淫羊藿苷可显著缩短大/小鼠的强迫游泳不动时间和小鼠悬尾不动时间，与对照组相比差异有统计学意义（P <0.05）；在皮质酮损伤PC12细胞的模型上，淫羊藿苷可显著提高PC12细胞的存活率，拮抗皮质酮诱导的细胞损伤作用。结论：淫羊藿苷具有明显的抗抑郁效果，其抗抑郁作用与神经细胞保护作用有关。
목적：：연구음양곽감적항억욱급대피질동치PC12세포손상적보호작용。방법：채용대/소서강박유영、소서현미삼충실험모형，장동물수궤분위대조조、음양곽감저제량조、음양곽감고제량조、아미체림조，관찰약물대대/소서강박유영불동시간、소서현미불동시간적영향；병재세포수평건립피질동손상PC12세포모형，관찰음양곽감적세포보호작용。결과：재대/소서강박유영화소서현미실험중，음양곽감가현저축단대/소서적강박유영불동시간화소서현미불동시간，여대조조상비차이유통계학의의（P <0.05）；재피질동손상PC12세포적모형상，음양곽감가현저제고PC12세포적존활솔，길항피질동유도적세포손상작용。결론：음양곽감구유명현적항억욱효과，기항억욱작용여신경세포보호작용유관。
Objective:To investigate the antidepressant effect and the protective effect against corticosterone-induced PC12 cell neurotoxity of icariin. Methods:The antidepressant effects of icariin were evaluated by the tail suspension test in mice and the forced swimming tests in rats and mice. The animals were divided into four groups randomly:control group, low dose icariin group, high dose icariin group and amitriptyline group. The effects of icariin on immobility time were measured in the forced swimming test in rats and mice, together with the tail suspension test in mice. Likewise, PC12 cell injury model was induced by corticosterone and the protective effect of icariin on the cell viability was measured by MTT assay. Results:Icariin could signiifcantly decrease the immobility time in tail suspension test in mice, as well as the forced swimming tests in rats and mice, with the signiifcant differences when compared with the control group. In addition, icariin could improve the survival rate of PC12 cells injuried by corticosterone. Conclusion:Icariin possessed antidepressant effect and its antidepressant effect maybe involve in the protective effects of neural cells.