中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2013年
10期
977-983
,共7页
徐灿霞%万迎春%郭涛%陈雄
徐燦霞%萬迎春%郭濤%陳雄
서찬하%만영춘%곽도%진웅
硫化氢%幽门螺杆菌%CSE-1细胞%NF-κB%IL-8
硫化氫%幽門螺桿菌%CSE-1細胞%NF-κB%IL-8
류화경%유문라간균%CSE-1세포%NF-κB%IL-8
hydrogen sulifde%Helicobacter pylori%CSE-1 cell%NF-κB%IL-8
目的：观察硫化氢(hydrogen sulfide，H2S)对幽门螺杆菌(H. pylori)感染的GES-1细胞CSE，NF-κB及IL-8 mRNA表达及其形态学的影响，探讨其对H. pylori所致胃黏膜细胞炎症的作用及机制。方法：将GES-1细胞培养24 h后分为对照组(不加H. pylori及NaHS)、H. pylori组、NaHS组(又分为4个亚组，分别加入50，100，200或400μmol/L NaHS)和H. pylori + NaHS组(又分为4个亚组，分别加入H. pylori与50，100，200或400μmol/L NaHS)，每组分别培养3，6，及12 h，用RT-PCR法检测各组GES-1细胞CSE，NF-κB 及IL-8 mRNA表达，并分析其相关性。结果：H. pylori组CSE，NF-κB及IL-8 mRNA表达均较对照组增加(P<0.05)，200μmol/L NaHS组和400μmol/L NaHS组CSE表达较对照组降低(P<0.05)；而NaHS各组NF-κB和IL-8 mRNA表达与对照组比较差异无统计学意义(P>0.05)；NaHS各组、H. pylori +200μmol/L NaHS组及H. pylori +400μmol/L NaHS组CSE，NF-κB及IL-8 mRNA表达均较H. pylori组降低(P<0.05)；H. pylori组、H. pylori +200μmol/L NaHS组及H. pylori +400μmol/L NaHS组CSE，NF-κB及IL-8 mRNA表达之间均呈正相关(P<0.05)。结论：H. pylori诱导GES-1细胞NF-κB和IL-8 mRNA表达，并上调CSE mRNA表达；200和400μmol/L NaHS能抑制H. pylori感染诱导的GES-1细胞NF-κB和IL-8 mRNA表达，改善H. pylori感染所致的细胞形态学变化，对细胞起保护作用。
目的：觀察硫化氫(hydrogen sulfide，H2S)對幽門螺桿菌(H. pylori)感染的GES-1細胞CSE，NF-κB及IL-8 mRNA錶達及其形態學的影響，探討其對H. pylori所緻胃黏膜細胞炎癥的作用及機製。方法：將GES-1細胞培養24 h後分為對照組(不加H. pylori及NaHS)、H. pylori組、NaHS組(又分為4箇亞組，分彆加入50，100，200或400μmol/L NaHS)和H. pylori + NaHS組(又分為4箇亞組，分彆加入H. pylori與50，100，200或400μmol/L NaHS)，每組分彆培養3，6，及12 h，用RT-PCR法檢測各組GES-1細胞CSE，NF-κB 及IL-8 mRNA錶達，併分析其相關性。結果：H. pylori組CSE，NF-κB及IL-8 mRNA錶達均較對照組增加(P<0.05)，200μmol/L NaHS組和400μmol/L NaHS組CSE錶達較對照組降低(P<0.05)；而NaHS各組NF-κB和IL-8 mRNA錶達與對照組比較差異無統計學意義(P>0.05)；NaHS各組、H. pylori +200μmol/L NaHS組及H. pylori +400μmol/L NaHS組CSE，NF-κB及IL-8 mRNA錶達均較H. pylori組降低(P<0.05)；H. pylori組、H. pylori +200μmol/L NaHS組及H. pylori +400μmol/L NaHS組CSE，NF-κB及IL-8 mRNA錶達之間均呈正相關(P<0.05)。結論：H. pylori誘導GES-1細胞NF-κB和IL-8 mRNA錶達，併上調CSE mRNA錶達；200和400μmol/L NaHS能抑製H. pylori感染誘導的GES-1細胞NF-κB和IL-8 mRNA錶達，改善H. pylori感染所緻的細胞形態學變化，對細胞起保護作用。
목적：관찰류화경(hydrogen sulfide，H2S)대유문라간균(H. pylori)감염적GES-1세포CSE，NF-κB급IL-8 mRNA표체급기형태학적영향，탐토기대H. pylori소치위점막세포염증적작용급궤제。방법：장GES-1세포배양24 h후분위대조조(불가H. pylori급NaHS)、H. pylori조、NaHS조(우분위4개아조，분별가입50，100，200혹400μmol/L NaHS)화H. pylori + NaHS조(우분위4개아조，분별가입H. pylori여50，100，200혹400μmol/L NaHS)，매조분별배양3，6，급12 h，용RT-PCR법검측각조GES-1세포CSE，NF-κB 급IL-8 mRNA표체，병분석기상관성。결과：H. pylori조CSE，NF-κB급IL-8 mRNA표체균교대조조증가(P<0.05)，200μmol/L NaHS조화400μmol/L NaHS조CSE표체교대조조강저(P<0.05)；이NaHS각조NF-κB화IL-8 mRNA표체여대조조비교차이무통계학의의(P>0.05)；NaHS각조、H. pylori +200μmol/L NaHS조급H. pylori +400μmol/L NaHS조CSE，NF-κB급IL-8 mRNA표체균교H. pylori조강저(P<0.05)；H. pylori조、H. pylori +200μmol/L NaHS조급H. pylori +400μmol/L NaHS조CSE，NF-κB급IL-8 mRNA표체지간균정정상관(P<0.05)。결론：H. pylori유도GES-1세포NF-κB화IL-8 mRNA표체，병상조CSE mRNA표체；200화400μmol/L NaHS능억제H. pylori감염유도적GES-1세포NF-κB화IL-8 mRNA표체，개선H. pylori감염소치적세포형태학변화，대세포기보호작용。
Objective: To investigate the effect of hydrogen sulifde (H2S) on the expression of CSE, NF-κB, and IL-8 mRNA in GES-1 cells withHelicobacter pylori (H. pylori) infection and to explore its mechanism on gastric mucosa inlfammation caused byH. pylori. <br> Methods: GES-1 cells were cultured for 24 h and divided into a control group (neitherH. pylori nor NaHS), anH. pylori group, a NaHS group (which was further divided into 4 groups at 50, 100, 200, or 400 μmol/L NaHS), andH. pylori + NaHS group (which was further divided into 4 groups at 50, 100, 200, or 400 μmol/L NaHS). Each group was then cultured for 3, 6, or 12 h. The expression of CSE, NF-κB, and IL-8 mRNA was measured by RT-PCR, and their correlation was analyzed. Results: The expression of CSE, NF-κB, and IL-8 mRNA in GES-1 cells in theH. pylori group was higher than that in the control group. The expression of CSE in the 200 μmol/L NaHS group and 400 μmol/L NaHS group was lower than that of the control group (P<0.05), whereas the expression of NF-κB and IL-8 in all NaHS groups had no statistical differences compared with the control group (P>0.05). The expression of CSE, NF-κB, and IL-8 mRNA in all groups of NaHS,H. pylori + 200 μmol/L NaHS group, andH. pylori + 400 μmol/L NaHS group was lower than that in theH. pylori group (P<0.05). There was positive correlation among the expressions of CSE, NF-κB, and IL-8 mRNA in theH. pylori group, theH. pylori + 200 μmol/L NaHS group, and theH. pylori +400 μmol/L NaHS group (P<0.05). <br> Conclusion:H. pylori can induce NF-κB and IL-8 mRNA expression and upregulate CSE mRNA expression. At 200 and 400 μmol/L, NaHS can suppressH. pylori-induced NF-κB and IL-8 mRNA expression and ameliorate the morphology ofH. pylori-induced GES-1 injury, which may protect gastric epithelial cells byH. pylori infection.
