CAJ | 학술논문

应用胶体金免疫层析技术，采用柠檬酸三钠还原法制备胶体金颗粒，在玻璃纤维膜上包被胶体金标记PCV-2抗原（cap蛋白），在硝酸纤维素膜上检测线和对照线处分别包被PCV-2抗原（cap蛋白）和PCV-2单抗，制成猪圆环病毒2型抗体免疫金标检测试纸条。该试纸条与猪圆环2型抗体酶联免疫检测试剂盒对比，符合率为94．7％，整个实验过程只需15min，与ELISA试剂盒比较，具有操作方便、快速、直观的优点。
응용효체금면역층석기술，채용저몽산삼납환원법제비효체금과립，재파리섬유막상포피효체금표기PCV-2항원（cap단백），재초산섬유소막상검측선화대조선처분별포피PCV-2항원（cap단백）화PCV-2단항，제성저원배병독2형항체면역금표검측시지조。해시지조여저원배2형항체매련면역검측시제합대비，부합솔위94．7％，정개실험과정지수15min，여ELISA시제합비교，구유조작방편、쾌속、직관적우점。
Based on the principle of colloidal gold immunochromatography, with the tri - sodium citrate reoucuon method, colloidal gold strip was prepared and applied to detect the anti -PCV -2 antibody. PCV -2 and anti - PCV - 2 Monoclonal antibody was coated on the test region and control zone respectively. The accuracy of strip is 94.7% compared to ELISA test kit. The whole experimentation merely needed fifteen minutes. This test shows that this strip has the merits of convenient operation, celerity and intuitional.