CAJ | 학술논문

为寻求控制一种新的防控猪高热综合征中草药饲料添加剂质量的有效方法，本研究采用反相高效液相色谱指纹图谱技术评价中草药添加剂质量，并对其主要成分定量分析方法进行探讨。液相色谱的条件为：AgilentHC—C18色谱柱（4．6mm×250mm，5μm）和Agilent保护柱，柱温25℃，流速1mL/min，以乙腈-0．2％磷酸溶液为流动相进行梯度洗脱，检测波长为280nm。通过对黄芩苷、橙皮苷、甘草苷对照品和12批次中草药饲料添加剂RP—HPLC指纹图谱的分析，建立了该中草药添加剂RP—HPLC指纹图谱，获得包括有甘草苷、橙皮苷和黄芩苷等相对稳定的15个共有特征峰。定量分析表明每克中草药添加剂样品中平均含量黄芩苷为（8．488±1．084）mg，橙皮苷为（6．321±0．379）mg，甘草苷（0．218±0．157）mg。本研究建立的定性和定量检测方法可用于新的中草药添加剂的质量检测。
위심구공제일충신적방공저고열종합정중초약사료첨가제질량적유효방법，본연구채용반상고효액상색보지문도보기술평개중초약첨가제질량，병대기주요성분정량분석방법진행탐토。액상색보적조건위：AgilentHC—C18색보주（4．6mm×250mm，5μm）화Agilent보호주，주온25℃，류속1mL/min，이을정-0．2％린산용액위류동상진행제도세탈，검측파장위280nm。통과대황금감、등피감、감초감대조품화12비차중초약사료첨가제RP—HPLC지문도보적분석，건립료해중초약첨가제RP—HPLC지문도보，획득포괄유감초감、등피감화황금감등상대은정적15개공유특정봉。정량분석표명매극중초약첨가제양품중평균함량황금감위（8．488±1．084）mg，등피감위（6．321±0．379）mg，감초감（0．218±0．157）mg。본연구건립적정성화정량검측방법가용우신적중초약첨가제적질량검측。
In order to find an effective method to control the quality of a novel Chinese herbal additive to control the porcine hyperpyrexia syhdrome, a RP - HPLC fingerprint has been developed. The conditions were as follows ： Using Agilent HC - C18 column （4.6mm×250mm,5μm） with the gradient elution program. The mobile phase was composed of acetonitrile and 0.2% phosphate. The column temperature is 25℃. The flow rate was kept at 1.0 mL/min - 1 and the detection wavelength was 280 nm. Analysis the RP - HPLC result of reference substance of baicalin, hesperidin, liquiritin and 12 batches of herbal feed additives, the RP -HPLC fingerprint common pattern of the chinese herbal additives was established. Compared with the reference substance, 15 common peaks were analyzed. Average content per gram of Chinese herbal medicine additive samples are baicalin 8. 488±1. 084 mg, hesperidin 6.321 ±0.379 nag, liquiritin 0.218±0. 157 nag. This method could used for valuing control the quality of the additives.