草原与草坪
草原與草坪
초원여초평
GRASSLAND AND TURF
2012年
2期
15-19
,共5页
安惠惠%马晖玲%白生军%马祥
安惠惠%馬暉玲%白生軍%馬祥
안혜혜%마휘령%백생군%마상
匍匐翦股颖%2,4-D%作用时间%分化
匍匐翦股穎%2,4-D%作用時間%分化
포복전고영%2,4-D%작용시간%분화
creeping bentgrass%2%4-D%treatment duration%differentiation
以匍匐翦股颖Penn A-1和Penn A-4为供试品种,就2,4-D不同浓度和作用时间对匍匐翦股颖愈伤组织诱导及分化的影响进行试验。结果表明,2mg/L的2,4-D对Penn A-1愈伤组织诱导效果最佳,出愈率为36.4%,4mg/L的2,4-D对Penn A-4的效果最佳,出愈率为82.4%;愈伤组织最佳分化时间为2,4-D作用0～30d时,Penn A-1和Penn A-4分化率分别保持在50%和83%;分化能力随2,4-D作用时间的增长而下降,而颗粒状、质地松散的愈伤组织最终将失去分化能力。
以匍匐翦股穎Penn A-1和Penn A-4為供試品種,就2,4-D不同濃度和作用時間對匍匐翦股穎愈傷組織誘導及分化的影響進行試驗。結果錶明,2mg/L的2,4-D對Penn A-1愈傷組織誘導效果最佳,齣愈率為36.4%,4mg/L的2,4-D對Penn A-4的效果最佳,齣愈率為82.4%;愈傷組織最佳分化時間為2,4-D作用0～30d時,Penn A-1和Penn A-4分化率分彆保持在50%和83%;分化能力隨2,4-D作用時間的增長而下降,而顆粒狀、質地鬆散的愈傷組織最終將失去分化能力。
이포복전고영Penn A-1화Penn A-4위공시품충,취2,4-D불동농도화작용시간대포복전고영유상조직유도급분화적영향진행시험。결과표명,2mg/L적2,4-D대Penn A-1유상조직유도효과최가,출유솔위36.4%,4mg/L적2,4-D대Penn A-4적효과최가,출유솔위82.4%;유상조직최가분화시간위2,4-D작용0～30d시,Penn A-1화Penn A-4분화솔분별보지재50%화83%;분화능력수2,4-D작용시간적증장이하강,이과립상、질지송산적유상조직최종장실거분화능력。
The effect of different 2,4-D concentrations and time on callus induction and differentiation of two creeping bentgrass cultivars（Penn A-1 and Penn A-4）were studied.The result indicated that the optimum 2,4-D concentrations for callus induction of Penn A-1and Penn A-4 were 2 mg/L and 4 mg/L,and the callus generation rates were 36.4% and 82.4% respectively.The optimum treatment duration for callus differentiation ranged from 0 to 30 days,and the differentiation rates of Penn A-1and Penn A-4 were 50% and 83%,respectively.Differentiation rate decreased as the increase of 2,4-D treatment duration,and the particle,loose calli that lost differentiation capability were found.
