CAJ | 학술논문

目的：分析酒精性肝病患者血浆白细胞介素-21（IL-21）水平及重组IL-21体外对LX-2肝星状细胞增殖和活化的影响。方法采用ELISA法检测17例酒精性肝炎、51例酒精性肝硬化患者和20例健康人血浆IL-21水平；体外培养LX-2肝星状细胞，以IL-21（1ng/ml或10ng/ml）处理24 h或48 h，检测LX-2细胞增殖及α-平滑肌肌动蛋白表达。结果与健康对照人群比，酒精性肝炎和酒精性肝硬化患者血浆IL-21水平均显著升高（P<0.05），但酒精性肝炎和肝硬化患者之间无显著性差异，不同Child分级的肝硬化患者之间也无显著性差异；在IL-21作用24～48 h后，LX-2细胞增殖水平与对照组比无显著性差异，但α-平滑肌肌动蛋白表达均较对照组显著升高。结论血浆IL-21可能通过促进肝星状细胞活化参与了酒精性肝病患者肝纤维化的发生和发展。
목적：분석주정성간병환자혈장백세포개소-21（IL-21）수평급중조IL-21체외대LX-2간성상세포증식화활화적영향。방법채용ELISA법검측17례주정성간염、51례주정성간경화환자화20례건강인혈장IL-21수평；체외배양LX-2간성상세포，이IL-21（1ng/ml혹10ng/ml）처리24 h혹48 h，검측LX-2세포증식급α-평활기기동단백표체。결과여건강대조인군비，주정성간염화주정성간경화환자혈장IL-21수평균현저승고（P<0.05），단주정성간염화간경화환자지간무현저성차이，불동Child분급적간경화환자지간야무현저성차이；재IL-21작용24～48 h후，LX-2세포증식수평여대조조비무현저성차이，단α-평활기기동단백표체균교대조조현저승고。결론혈장IL-21가능통과촉진간성상세포활화삼여료주정성간병환자간섬유화적발생화발전。
Objective To evaluate plasma IL-21 levels in patients with alcoholic liver diseases and the ef-fects of recombinant IL-21 on proliferation and activation of LX-2 hepatic stellate cells in vitro. Methods The plasma levels of IL-21 in patients with alcoholic hepatitis(n=17),patients with alcoholic liver cirrhosis(n=51),and healthy controls (n=20) were measured by ELISA;the LX-2 hepatic stellate cells were subjected to recombinant IL-21 at the concentration of 1 ng/ml or 10 ng/ml for 24 or 48 hours,then the cell proliferation and expression of α-smooth muscle actin were determined by CCK-8 and by FACs,respectively. Results Plasma levels of IL-21 were significantly elevated in patients with alcoholic hepatitis and cirrhosis as compared to that in healthy controls (P<0.05),however,no significant difference was observed between patients with alcoholic hepatitis and patients with alcoholic cirrhosis,and no significant difference was found among cirrhotic patients with different Child class either;Incubation of LX-2 hepatic stellate cells with IL-21 at 1 ng/ml or 10 ng/ml for 24 or 48 hours did not affect cell proliferation,but significantly increased the expression of α-smooth muscle actin in the cells (P<0.05). Conclusion IL-21 might contribute to the fibrogenesis in alcoholic liver diseases though induction of activation of hepatic stellate cells.