实用肝脏病杂志
實用肝髒病雜誌
실용간장병잡지
JOURNAL OF CLINICAL HEPATOLOGY
2014年
1期
15-17
,共3页
张军会%王永辉%周然%王悦尧
張軍會%王永輝%週然%王悅堯
장군회%왕영휘%주연%왕열요
酒精性脂肪肝%肠源性内毒素血症%肠黏膜通透性%大鼠
酒精性脂肪肝%腸源性內毒素血癥%腸黏膜通透性%大鼠
주정성지방간%장원성내독소혈증%장점막통투성%대서
Alcoholic fatty liver%Intestinal endotoxemia%Permeability of intestinal mucosa%Rats
目的：建立酒精性脂肪肝大鼠肠源性内毒素血症模型。方法采用梯度酒精灌胃法早晚两次灌胃，并以10%酒精为饮料，建立大鼠酒精性脂肪肝模型。分别于3w和6w检测肝脂肪变、肝内炎症、肝功能和血清内毒素水平。结果模型组自6w出现显著的肝脂肪变，肝指数（3.6±0.2）、肝脂肪变积分（3.0±0.9）和炎症积分（1.0±0.6）均显著高于同期对照组水平[分别为（3.1±0.1）、（0.0±0.0）和（0.0±0.0），P＜0.05]；在6周时模型动物血浆内毒素、血清D-乳酸、二胺氧化酶和AST分别为（1435.6±52.9）pg/ml、（20.7±5.4）mmol/L、（25.5±2.0）U/L和（124.5±13.2） U/L，较正常组均明显升高[分别为（89.9±10.5）pg/ml、（5.0±1.1）mmol/L、（7.4±1.7）U/L和（40.4±15.2）U/L，P＜0.05]。结论用该方法连续6w成功建立单纯性酒精性脂肪肝肠源性内毒素血症模型。
目的：建立酒精性脂肪肝大鼠腸源性內毒素血癥模型。方法採用梯度酒精灌胃法早晚兩次灌胃，併以10%酒精為飲料，建立大鼠酒精性脂肪肝模型。分彆于3w和6w檢測肝脂肪變、肝內炎癥、肝功能和血清內毒素水平。結果模型組自6w齣現顯著的肝脂肪變，肝指數（3.6±0.2）、肝脂肪變積分（3.0±0.9）和炎癥積分（1.0±0.6）均顯著高于同期對照組水平[分彆為（3.1±0.1）、（0.0±0.0）和（0.0±0.0），P＜0.05]；在6週時模型動物血漿內毒素、血清D-乳痠、二胺氧化酶和AST分彆為（1435.6±52.9）pg/ml、（20.7±5.4）mmol/L、（25.5±2.0）U/L和（124.5±13.2） U/L，較正常組均明顯升高[分彆為（89.9±10.5）pg/ml、（5.0±1.1）mmol/L、（7.4±1.7）U/L和（40.4±15.2）U/L，P＜0.05]。結論用該方法連續6w成功建立單純性酒精性脂肪肝腸源性內毒素血癥模型。
목적：건립주정성지방간대서장원성내독소혈증모형。방법채용제도주정관위법조만량차관위，병이10%주정위음료，건립대서주정성지방간모형。분별우3w화6w검측간지방변、간내염증、간공능화혈청내독소수평。결과모형조자6w출현현저적간지방변，간지수（3.6±0.2）、간지방변적분（3.0±0.9）화염증적분（1.0±0.6）균현저고우동기대조조수평[분별위（3.1±0.1）、（0.0±0.0）화（0.0±0.0），P＜0.05]；재6주시모형동물혈장내독소、혈청D-유산、이알양화매화AST분별위（1435.6±52.9）pg/ml、（20.7±5.4）mmol/L、（25.5±2.0）U/L화（124.5±13.2） U/L，교정상조균명현승고[분별위（89.9±10.5）pg/ml、（5.0±1.1）mmol/L、（7.4±1.7）U/L화（40.4±15.2）U/L，P＜0.05]。결론용해방법련속6w성공건립단순성주정성지방간장원성내독소혈증모형。
Objective To establish an experimental model of alcoholic fatty liver accompanied with intesti-nal endotoxemia in rats. Methods A rat model of alcoholic fatty liver was established by gradient ethanol gavage method;The liver steatosis,intrahepatic inflammation,liver function tests and serum lipopolysaccharide were deter-mined at 3 and 6 weeks of experiment. Result Significant liver steatosis was observed after 6 weeks of ethanol administration in the model rats;The liver index,liver steatosis scores,inflammation scores in model rats were(3.6± 0.2),(3.0±0.9)and (1.0±0.6),respectively, significantly higher than those in normal controls [(3.1±0.1),(0.0±0.0) and (0.0 ±0.0),respectively,P<0.05)];Similarly,serum lipopolysaccharide,D-lactate,diamine oxidase and aspertate aminotransferase levels in model rats at 6 months were (1435.6 ±52.9) pg/ml,(20.7 ±5.4) mmol/L,(25.5 ±2.0)U/L and (124.5±13.2) U/L,which were also significantly higher than those in normal controls[(89.9±10.5) pg/ml,(5.0± 1.1)mmol/L,(7.4±1.7) U/L and(40.4±15.2) U/L,respectively,P<0.05]. Conclusion A rat model of alcoholic fatty liver accompanied with intestinal endotoxemia can be successfully establish by using gradient ethanol gavage for 6 weeks.
