CAJ | 학술논문

目的：探讨丹参酮ⅡA（ TanⅡA）对晚期糖化终末产物（ AGE）诱导人肾小球系膜细胞（ HMCs）的晚期糖化终末产物受体（ RAGE）表达和氧化应激水平的影响。方法常规方法培养HMCs，分为：对照组；AGE组〔晚期糖化终末产物牛血清清蛋白（ AGE -BSA ）1.0、10.0、50.0、100.0μg/ml〕；AGE +TanⅡA 组（ AGE -BSA 50.0μg/ml，TanⅡA 0、0.1、1.0、5.0、10.0μg/ml），以GAPDH为内参照，分别采用Western blotting和实时定量PCR法检测RAGE和 mRNA 表达水平，同时检测细胞培养上清液超氧化物歧化酶（ SOD ）、谷胱甘肽过氧化物酶（ GSH-Px）和丙二醛（ MDA）水平。结果对照组与不同浓度AGE组HMCs RAGE和mRNA表达比较，差异均有统计学意义（F 值分别为4.428和5.031，P <0.05）；其中与对照组比较，10.0、50.0、100.0μg/ml AGE 组 HMCs RAGE和mRNA表达水平升高（P<0.05）。对照组与AGE+不同浓度TanⅡA组HMCs RAGE和mRNA表达比较有差异（F值为5.002和5.312，P<0.05）；其中与AGE+0μg/ml TanⅡA组比较，对照组、AGE+0.1μg/ml TanⅡA组、AGE+1.0μg/ml TanⅡA组、AGE+5.0μg/ml TanⅡA组、AGE+10.0μg/ml TanⅡA组HMCs RAGE和mRNA表达降低（P<0.05）。对照组与不同浓度AGE组及AGE+TanⅡA组上清液SOD、GSH-Px和MDA水平比较有差异（P<0.05）。结论 TanⅡA能够明显降低AGE诱导HMCs RAGE和mRNA的表达水平，同时氧化应激水平也得到明显改善。
목적：탐토단삼동ⅡA（ TanⅡA）대만기당화종말산물（ AGE）유도인신소구계막세포（ HMCs）적만기당화종말산물수체（ RAGE）표체화양화응격수평적영향。방법상규방법배양HMCs，분위：대조조；AGE조〔만기당화종말산물우혈청청단백（ AGE -BSA ）1.0、10.0、50.0、100.0μg/ml〕；AGE +TanⅡA 조（ AGE -BSA 50.0μg/ml，TanⅡA 0、0.1、1.0、5.0、10.0μg/ml），이GAPDH위내삼조，분별채용Western blotting화실시정량PCR법검측RAGE화 mRNA 표체수평，동시검측세포배양상청액초양화물기화매（ SOD ）、곡광감태과양화물매（ GSH-Px）화병이철（ MDA）수평。결과대조조여불동농도AGE조HMCs RAGE화mRNA표체비교，차이균유통계학의의（F 치분별위4.428화5.031，P <0.05）；기중여대조조비교，10.0、50.0、100.0μg/ml AGE 조 HMCs RAGE화mRNA표체수평승고（P<0.05）。대조조여AGE+불동농도TanⅡA조HMCs RAGE화mRNA표체비교유차이（F치위5.002화5.312，P<0.05）；기중여AGE+0μg/ml TanⅡA조비교，대조조、AGE+0.1μg/ml TanⅡA조、AGE+1.0μg/ml TanⅡA조、AGE+5.0μg/ml TanⅡA조、AGE+10.0μg/ml TanⅡA조HMCs RAGE화mRNA표체강저（P<0.05）。대조조여불동농도AGE조급AGE+TanⅡA조상청액SOD、GSH-Px화MDA수평비교유차이（P<0.05）。결론 TanⅡA능구명현강저AGE유도HMCs RAGE화mRNA적표체수평，동시양화응격수평야득도명현개선。
Objective To investigate the effect of Tanshinone ⅡA( TanⅡA)on the expression of receptor for ad-vanced glycated endproducts( RAGE)and the oxidative stress status of human mesangial cells induced by AGE. Methods Hu-man mesangial cells(HMCs)were cultured with various concentration of AGE-BSA(1. 0 μg/ml,10. 0 μg/ml,50. 0 μg/ml and 100. 0 μg/ml),and then were treated with Tan ⅡA(0. 1 μg/ml,1. 0 μg/ml,5. 0 μg/ml and 10. 0 μg/ml). The cul-tured HMCs were divided into AGE group,AGE+TanⅡA group and control group. By using GAPDH as reference,the protein and mRNA expression of RAGE were evaluated by Western blotting analysis and real-time PCR respectively. The activity of su-peroxide dismutase( SOD),glutathione peroxidase( GSH-Px)and malonaldehyde( MDA)levels in the supernatant of HMCs were measured. Results The expressions of RAGE and mRNA in HMCs between control group and different concentration of AGE groups all showed statistically significant differences(F=4. 428 and 5. 031,P<0. 05). Compared with the control group,the expressions of RAGE and mRNA in HMCs of 10. 0 μg/ml,50. 0 μg/ml and 100. 0 μg/ml AGE groups were significantly higher (P<0. 05). The expressions of RAGE and mRNA in HMCs between control group and AGE+different concentrations of TanⅡA groups all showed statistically significant differences(F=5. 002 and 5. 312,P<0. 05). Compared with AGE+0 μg/ml Tan ⅡA group,the expressions of RAGE and mRNA in HMCs of the control group,AGE+0. 1 μg/ml TanⅡA group,AGE+1. 0μg/ml TanⅡA group,AGE+5. 0 μg/ml TanⅡA group and AGE+10. 0 μg/ml TanⅡA group were significantly lower( P<0. 05). The SOD,GSH-Px and MDA levels in the supernantant of HMCs showed statistically significant differences between the control group,different concentrations of AGE group and AGE+TanⅡA group(P<0. 05). Conclusion TanⅡA could sig-nificantly reduce the RAGE and mRNA expression in HMCs induced by AGE,and the oxidative stress levels are also improved.