CAJ | 학술논문

目的：对体外培养的人大骨节病和骨关节炎软骨细胞添加不同剂量透明质酸，观察干预前后软骨细胞透明质酸合成酶2（HAS2）mRNA的变化。方法选取2006年8月-2008年7月陕西地方病研究所明确诊断大骨节病，并实施膝关节游离体摘除术后取出游离体及关节软骨6例为大骨节病组；西安市红十字会医院明确诊断骨关节炎，并行全膝关节置换术后的膝关节软骨6例为骨关节炎组；另选择同时期由于意外车祸等原因截肢或身亡者的新鲜膝关节软骨6例为对照组。对3组软骨细胞采用不同剂量的透明质酸进行干预，分为0μg/ml（H0）、100μg/ml（H100）、500μg/ml（H500），通过RT-PCR法观察透明质酸对3组软骨细胞HAS2mRNA表达的影响。结果干预前3组HAS2mRNA表达比较，差异有统计学意义（P<0.05）；其中骨关节炎组较对照组降低（P<0.05）。干预后3组不同剂量间HAS2mRNA表达比较，差异均无统计学意义（P>0.05）。结论退变的大骨节病和骨关节炎软骨细胞合成透明质酸能力下降；应用外源性透明质酸后，有增加软骨细胞自身透明质酸合成的趋势，为透明质酸关节腔注射治疗大骨节病及骨关节炎提供了理论基础。
목적：대체외배양적인대골절병화골관절염연골세포첨가불동제량투명질산，관찰간예전후연골세포투명질산합성매2（HAS2）mRNA적변화。방법선취2006년8월-2008년7월협서지방병연구소명학진단대골절병，병실시슬관절유리체적제술후취출유리체급관절연골6례위대골절병조；서안시홍십자회의원명학진단골관절염，병행전슬관절치환술후적슬관절연골6례위골관절염조；령선택동시기유우의외차화등원인절지혹신망자적신선슬관절연골6례위대조조。대3조연골세포채용불동제량적투명질산진행간예，분위0μg/ml（H0）、100μg/ml（H100）、500μg/ml（H500），통과RT-PCR법관찰투명질산대3조연골세포HAS2mRNA표체적영향。결과간예전3조HAS2mRNA표체비교，차이유통계학의의（P<0.05）；기중골관절염조교대조조강저（P<0.05）。간예후3조불동제량간HAS2mRNA표체비교，차이균무통계학의의（P>0.05）。결론퇴변적대골절병화골관절염연골세포합성투명질산능력하강；응용외원성투명질산후，유증가연골세포자신투명질산합성적추세，위투명질산관절강주사치료대골절병급골관절염제공료이론기출。
Objective To investigate the effect of hyluronic acid(HA)on the expression of HAS2's mRNA in chon-drocytes of Kashin-beck disease( KBD)and osteoarthritis( OA)cultured in vitro. Methods A total of 6 patients who were di-agnosed with KBD in Shanxi Provincial Institute for Endemic Disease from August 2006 to July 2008 , were selected as KBD group,the loose bodies and articular cartilage of KBD patients were removed through surgery. A total of 6 patients who were diag-nosed with OA in Xi'an City Red Cross Hospital,were selected as OA group,the knee articular cartilage of OA patients were re-moved through total knee arthroplasty. At the same time,amputees and the dead(6 cases)due to accidents such as traffic acci-dent,were selected as control group. Cells from 3 groups were treated with different doses of HA(0 μg/ml,100 μg/ml and 500μg/ml),the effect of HA on the expression of HAS2's mRNA in 3 groups of chondrocytes was detected by RT-PCR. Results Before treatment,there were significant differences in the expression levels of HAS2's mRNA among 3 groups of chondrocytes(P<0. 05). The HAS2's mRNA expression level in OA group was significantly lower than that in control group(P<0. 05). After treatment,there was no significant difference in the expression levels of HAS2's mRNA among different dose groups(P>0. 05). Conclusion The capacity of HA synthesis decreases in chondrocytes of KBD and OA. The exogenous HA can enhance chondro-cytes' capacity of HA synthesis,which provides the theory foundation for treating KBD and OA with intraarticular injection of HA.