CAJ | 학술논문

对冻墨鱼中分离的菌株 C6-1进行种属鉴定，并分析其低温状态下脂肪酸组成变化。通过VITEK 2 Compact全自动微生物鉴定仪分析菌株的生理生化特征；16S rRNA基因序列同源性分析确定该菌株的系统发育学地位；通过气相色谱和质谱联用法分析菌株的全细胞脂肪酸。菌株C6-1为革兰氏阴性菌，对弧菌抑制剂O／129敏感；生理生化特征与溶藻弧菌的相似性为98％，16S rRN A 序列与溶藻弧菌（GenBank No ．JF836167）的相似性为99％；菌株的主要脂肪酸组成为C12：0，C14：0，C16：1（n-7），2甲基-C17：0，C18：0和C18：1（n-9）；低温培养状态下，短链饱和脂肪酸（碳链长度小于16）相对含量由14．8％增至44．1％。
대동묵어중분리적균주 C6-1진행충속감정，병분석기저온상태하지방산조성변화。통과VITEK 2 Compact전자동미생물감정의분석균주적생리생화특정；16S rRNA기인서렬동원성분석학정해균주적계통발육학지위；통과기상색보화질보련용법분석균주적전세포지방산。균주C6-1위혁란씨음성균，대호균억제제O／129민감；생리생화특정여용조호균적상사성위98％，16S rRN A 서렬여용조호균（GenBank No ．JF836167）적상사성위99％；균주적주요지방산조성위C12：0，C14：0，C16：1（n-7），2갑기-C17：0，C18：0화C18：1（n-9）；저온배양상태하，단련포화지방산（탄련장도소우16）상대함량유14．8％증지44．1％。
Bacterial strain C6‐1 isolated from cuttlefish Sepiella maindroni was characterized and changes in membrane fatty acid composition of the bacterial strain C 6‐1 in response to low temperature were also studied . The physiological and biochemical characteristics were analyzed using VITEK 2 compact automated microbiology system . The 16S rRNA gene was sequenced and subjected to phylogenetic analysis ,and the fatty acids were detected by gas chromatography‐mass spectrometry (GC‐MS) .It was found that strain C6‐1 was Gram‐negative and susceptible to the vibriostatic agent O/129 ,that exhibited the maximal level of 98% probability to Vibrio alginolyticus based on the conventional physiological test . The sequence analysis of 16S rRNA gene of C6‐1 isolation showed that C6‐1 was very close to V . alginolyticus (GenBank No .JF836167) with similarity of 99% .The major fatty acids were shown to be C12 :0 ,C14:0 ,C16 :0 ,C16:1(n‐7) ,iso‐C17 :0 ,C18 :0 and C18 :1 (n‐9) .The major change in fatty acid composition in response to low temperature was characterized by increase in fatty acids with chain lengths less than 16 carbons from 14 .8% to 44 .1% .