CAJ | 학술논문

为探究海带凝集素最佳提取方案，分别对pH、料液比、浸泡时间、烘干温度4种单因素对海带凝集素提取的影响进行研究，缓冲液选取PBS、TBS和甘氨酸，料液比选取6个梯度（1∶5～1∶30），浸泡时间选取6个梯度（5～30h），烘干温度选取9个梯度（30～110℃），采取控制其中3个因素条件不变的方法，得到每个因素的最佳提取效果。其单因素最佳效果是pH缓冲液为TBS7．7，料液比1∶30，浸泡时间15h和烘干温度40、60℃。然后由各单因素中选出提取的最适条件及与最适条件相临的2个水平（共3个水平）进行四因素三水平的正交试验，根据正交试验极差分析法对正交试验数据进行分析，温度为50℃、缓冲液为TBSpH7．7、料液比为1∶20、浸泡时间为20h的组合对海带凝集素提取效果最好。从而得出一种提取海带凝集素最优的组合，使提取工艺得到优化。为海带凝集素的研究提供了理论基础。
위탐구해대응집소최가제취방안，분별대pH、료액비、침포시간、홍간온도4충단인소대해대응집소제취적영향진행연구，완충액선취PBS、TBS화감안산，료액비선취6개제도（1∶5～1∶30），침포시간선취6개제도（5～30h），홍간온도선취9개제도（30～110℃），채취공제기중3개인소조건불변적방법，득도매개인소적최가제취효과。기단인소최가효과시pH완충액위TBS7．7，료액비1∶30，침포시간15h화홍간온도40、60℃。연후유각단인소중선출제취적최괄조건급여최괄조건상림적2개수평（공3개수평）진행사인소삼수평적정교시험，근거정교시험겁차분석법대정교시험수거진행분석，온도위50℃、완충액위TBSpH7．7、료액비위1∶20、침포시간위20h적조합대해대응집소제취효과최호。종이득출일충제취해대응집소최우적조합，사제취공예득도우화。위해대응집소적연구제공료이론기출。
To further probe the best extract solution of lectin from L aminaria j aponica .In this study ,we separately selsct four kinds of single factor :pH ,Material‐water ratio ,soaking time and temperature .Buffer select PBS ,TBS and mannitol ,Material‐water ratio selected six gradient (1∶5—1∶30) ,soaking time select six gradient (5 -30h) ,temperature select nine gradient (30 -110 ℃) ,which adopt a method of controlling the three factors being equal ,to get the best extraction effect of each factor .Optimum results are buffer of TBS pH 7 .7 ,Material‐water ratio of 1∶30 ,soaking time for 15h and temperature of 40 ℃ and 60 ℃ .Elected from each single factor that the optimal conditions of extraction and two adjacent levels (three levels ) of it ,to make the four factors and three levels of orthogonal test . According to the orthogonal test range analysis ,buffer of TBS pH 7 .7 ,Material‐water ratio of 1∶20 ,soaking time for 20h , the temperature of the 50℃ is this best combination of each factor for extracting lectin from L aminaria j aponica .We get an optimum combination for extraction of lectin from L aminaria j aponica ,make the extraction process has been optimized .It provide a favorable theoretical basis for research of lectin from L aminaria j aponica .