CAJ | 학술논문

基于表面增强拉曼光谱的成像分析方法具有频带窄，水溶液背景弱，稳定性好，高特异性等优势已成为生物成像领域的优良选择。拉曼成像技术拓展了拉曼光谱的应用范围，使其不再只是检测单点化学成分的手段，而进一步用于对评价区域内化学物质成分、分布及变化进行整体统计和描述。本文探讨了表面增强拉曼散射的原理及增强机制，介绍了基于表面增强拉曼光谱的拉曼成像技术，并对其在无标记成像及带标记成像中的细胞成像、活体成像，特别是其在生物医学方面的应用进行了详细论述，最后讨论了表面增强拉曼光谱生物成像技术存在的问题，展望了该项技术的研究和应用前景。
기우표면증강랍만광보적성상분석방법구유빈대착，수용액배경약，은정성호，고특이성등우세이성위생물성상영역적우량선택。랍만성상기술탁전료랍만광보적응용범위，사기불재지시검측단점화학성분적수단，이진일보용우대평개구역내화학물질성분、분포급변화진행정체통계화묘술。본문탐토료표면증강랍만산사적원리급증강궤제，개소료기우표면증강랍만광보적랍만성상기술，병대기재무표기성상급대표기성상중적세포성상、활체성상，특별시기재생물의학방면적응용진행료상세논술，최후토론료표면증강랍만광보생물성상기술존재적문제，전망료해항기술적연구화응용전경。
The bioimaging technology based on the Surface-enhanced Raman Spectroscopy (SERS) has e-merged as a potential alternative due to its narrow bandwidth , weak background interference , high stability of label agent and specificity.Raman imaging technique can not only analyze a single point of the sample , but also offer an integral description of ingredient , distribution and variation for a large scale selected region .This paper introduces the principle of surface enhanced Raman scattering and its enhancement mechanism .Then, it reviews the recent development of SERS based bioimaging where both non-labeled and labelled SERS ima-ging techniques are included, and describes its applications to the biomedicine .Finally, it discusses some problems existed in the current technology and points out the future developing trends of the SERS -based bio-imaging.