CAJ | 학술논문

目的建立一种简便、稳定的大鼠原位节段小肠移植模型.方法供、受体均为雄性SD大鼠,各40只,采用供体肠系膜上动脉-腹主动脉漏斗状袖片与受体肾下腹主动脉端侧吻合,供体门静脉与受体左肾静脉Cuff套管袖套吻合.切除受体大部分小肠,供体小肠近、远端分别与受体残留小肠近、远端行端端吻合.结果供体手术时间(40 ±5)min,受体手术时间(50 ±8) min.热缺血时间(5±2)min,冷缺血时间(15±5) min.动脉吻合时间(5±2)min,静脉吻合时间(4 ±2)min.90.0％ (36/40)的大鼠术后存活时间＞10d.结论该模型操作简便,手术时间短,模型成活率高,稳定性好.
목적 건립일충간편、은정적대서원위절단소장이식모형.방법 공、수체균위웅성SD대서,각40지,채용공체장계막상동맥-복주동맥루두상수편여수체신하복주동맥단측문합,공체문정맥여수체좌신정맥Cuff투관수투문합.절제수체대부분소장,공체소장근、원단분별여수체잔류소장근、원단행단단문합.결과 공체수술시간(40 ±5)min,수체수술시간(50 ±8) min.열결혈시간(5±2)min,랭결혈시간(15±5) min.동맥문합시간(5±2)min,정맥문합시간(4 ±2)min.90.0％ (36/40)적대서술후존활시간＞10d.결론 해모형조작간편,수술시간단,모형성활솔고,은정성호.
Objective To establish a simple and stable orthotopic segmental small bowel transplantation model in rats.Methods Eighty male SD rats were divided into donors and recipients.Orthotopic segmental small bowel transplantation was performed by end-to-side anastomosis between donor abdominal aorta tundish-shape patch with arteria mesenterica superior pedicle and abdominal aorta of the recipients; end-to-end anastomosis between the portal vein of the donors and the left renal vein of the recipients was done using the cuff technique ; the large part of the small bowel of the recipients was excised,and it was replaced by the segmental intestine of the donors.Results The operation time of the donors and recipients were (40 ± 5) minutes and (50 ±8)minutes,respectively.The warm ischemia time and cold ischemia time were (5 ± 2) minutes and (15 ± 5) minutes,respectively.The anastomosis time of arteries and veins were (5 ± 2) minutes and (4 ± 2) minutes,respectively.The survival time of 90.0％ (36/40) of rats was more than 10 days.Conclusion The modified rat model of orthotopic segmental small bowel transplantation is easy to manipulate,and has the advantages of short operation time,high survival rate and stability.