食用菌学报
食用菌學報
식용균학보
ACTA EDULIS FUNGI
2014年
1期
42-46
,共5页
冯嫣%王家东%冯翠萍%常明昌%程红艳%孟俊龙
馮嫣%王傢東%馮翠萍%常明昌%程紅豔%孟俊龍
풍언%왕가동%풍취평%상명창%정홍염%맹준룡
灵芝粗多糖%肝组织形态%原癌基因%抑癌基因
靈芝粗多糖%肝組織形態%原癌基因%抑癌基因
령지조다당%간조직형태%원암기인%억암기인
Ganoderma lucidum polysaccharide (GP )%liver tissue morphology%proto-oncogene%tumor suppressor gene
采用苏木精-伊红染色法(hematoxylin-eosin staining )和实时荧光定量 PCR(real-time PCR,RT-PCR)法,研究灵芝(Ganoderma lucidum)粗多糖对二乙基亚硝胺(diethylnitrosamine,DEN)诱导的小鼠肝脏组织形态以及原癌基因H-ras和myc 和抑癌基因P53、Rb1 mRNA的影响,结果表明：灵芝粗多糖对 DEN 引起的小鼠肝脏损伤具有缓解作用,并可以抑制原癌基因的表达、提高抑癌基因的表达。
採用囌木精-伊紅染色法(hematoxylin-eosin staining )和實時熒光定量 PCR(real-time PCR,RT-PCR)法,研究靈芝(Ganoderma lucidum)粗多糖對二乙基亞硝胺(diethylnitrosamine,DEN)誘導的小鼠肝髒組織形態以及原癌基因H-ras和myc 和抑癌基因P53、Rb1 mRNA的影響,結果錶明：靈芝粗多糖對 DEN 引起的小鼠肝髒損傷具有緩解作用,併可以抑製原癌基因的錶達、提高抑癌基因的錶達。
채용소목정-이홍염색법(hematoxylin-eosin staining )화실시형광정량 PCR(real-time PCR,RT-PCR)법,연구령지(Ganoderma lucidum)조다당대이을기아초알(diethylnitrosamine,DEN)유도적소서간장조직형태이급원암기인H-ras화myc 화억암기인P53、Rb1 mRNA적영향,결과표명：령지조다당대 DEN 인기적소서간장손상구유완해작용,병가이억제원암기인적표체、제고억암기인적표체。
Sixty healthy,four week-old,mice were randomly divided into six groups (female∶male=1∶1 )as follows;normal control,diethylnitrosamine (DEN)model group,Ganoderma lucidum polysaccharide (GP) group,and low (DEN + 50 mg/kg·d),median (DEN+100 mg/kg·d)and high (DEN+200 mg/kg·d)GP dose groups.After 45 days,liver tissue morphology was observed using hematoxylin-eosin staining,and proto-oncogene (ras and H-myc)and tumor suppressor gene (p53 and Rb1)mRNA expression levels were determined using fluorescent quantitative real-time polymerase chain reaction (RT-PCR ). Our data demonstrated that GP had the protective function on liver cells of DEN-treated mice,and inhibited to a limited extent the occurrence or development of cancer by regulating the expression of proto-oncogenes and tumor suppressor genes.
