漳州职业技术学院学报
漳州職業技術學院學報
장주직업기술학원학보
JOURNAL OF ZHANGZHOU TECHNICAL INSTITUTE
2014年
2期
8-14
,共7页
紫菜%粗多糖%纤维素酶%果胶酶%二次通用旋转组合设计
紫菜%粗多糖%纖維素酶%果膠酶%二次通用鏇轉組閤設計
자채%조다당%섬유소매%과효매%이차통용선전조합설계
Porphyra yezoensis%crude polysaccharide%cellulase%pectinase%quadratic general rotary unitized design
采用复合酶法，在单因素基础上，通过二次通用旋转组合设计优化紫菜粗多糖（Crude Polysaccharide from Porphyra yezoensis，CPP）提取工艺条件。结果表明，紫菜粗多糖的最佳提取工艺条件为：料液比（mg/mL）1︰40、pH4.0，添加含5%纤维素酶和5%果胶酶的复合酶，65℃恒温酶解150min（纤维素酶30min、果胶酶120min），所得紫菜粗多糖提取率为4.78%。
採用複閤酶法，在單因素基礎上，通過二次通用鏇轉組閤設計優化紫菜粗多糖（Crude Polysaccharide from Porphyra yezoensis，CPP）提取工藝條件。結果錶明，紫菜粗多糖的最佳提取工藝條件為：料液比（mg/mL）1︰40、pH4.0，添加含5%纖維素酶和5%果膠酶的複閤酶，65℃恆溫酶解150min（纖維素酶30min、果膠酶120min），所得紫菜粗多糖提取率為4.78%。
채용복합매법，재단인소기출상，통과이차통용선전조합설계우화자채조다당（Crude Polysaccharide from Porphyra yezoensis，CPP）제취공예조건。결과표명，자채조다당적최가제취공예조건위：료액비（mg/mL）1︰40、pH4.0，첨가함5%섬유소매화5%과효매적복합매，65℃항온매해150min（섬유소매30min、과효매120min），소득자채조다당제취솔위4.78%。
With multiple enzyme method, the extraction conditions of crude polysaccharide from porphyra yezoensis (CPP) were optimized by the quadratic general rotary unitized design based on single factor. The result show that the optimum extraction conditions for CPP are as follow∶:410 solid -liquid ratio (mg/mL), added compound enzyme consisting of 5%cellulase for 30min and 5%pectinase for 120min at the condition of℃65 and pH4.0. Under these conditions, the extraction rate of CPP was 4.78%.
