CAJ | 학술논문

采用超声波、微波联合浸提方式提取海藻多酚，通过正交实验确立了多酚提取工艺条件，并以平板打孔抑制法测定了海藻多酚粗提物对7种细菌和4种真菌的抑菌活性。结果表明：采用料液比（匀浆：乙醇，g/mL）1：7，乙醇浓度85%，浸提温度70℃，浸提次数2次，浸提时间4 h，提取效果最佳，海带多酚提取率为2.08%。海藻多酚对供试的2种革兰氏阳性菌和5种革兰氏阴性菌都有一定程度的抑制活性，量效关系均呈剂量依赖型，最小抑菌浓度为10～40μg/mL。其中对大肠杆菌、绿脓杆菌抑制效果较明显，对青霉、白色念珠菌亦有抑制作用。
채용초성파、미파연합침제방식제취해조다분，통과정교실험학립료다분제취공예조건，병이평판타공억제법측정료해조다분조제물대7충세균화4충진균적억균활성。결과표명：채용료액비（균장：을순，g/mL）1：7，을순농도85%，침제온도70℃，침제차수2차，침제시간4 h，제취효과최가，해대다분제취솔위2.08%。해조다분대공시적2충혁란씨양성균화5충혁란씨음성균도유일정정도적억제활성，량효관계균정제량의뢰형，최소억균농도위10～40μg/mL。기중대대장간균、록농간균억제효과교명현，대청매、백색념주균역유억제작용。
Method of microwave and ultrasonic joint extraction of algae polyphenols was established by orthogonal experiment, and meanwhile antimicrobial activity of polyphenols was carried out in seven kinds of bacteria and four fungi by flat punch. The best process was solid-liquid ratio (homogenized:ethanol, g/mL) 1:7, 85% ethanol concentration, temperature 70 ℃, extraction 2 times, extraction 4 h, kelp polyphenols extraction rate 2.08%. Polyphenols had antimicrobial effects on 2 Gram-positive bacteria and five Gram-negative bacteri-a in a dose-dependent effect relationship type. The minimum inhibitory concentration of 10-40 μg/mL, which showed obvious inhibitory effect on E. coli, Pseudomonas aeruginosa, Penicillium, Candida albicans also inhib-ited.