CAJ | 학술논문

目的：观察低、中、高不同浓度中药怀牛膝加黄芪煎液对重型颅脑损伤大鼠脑组织含水量及水通道蛋白4（AQP4）表达的影响，探讨其治疗重型脑损伤性脑水肿最佳用药浓度及机制。方法将SD大鼠65只随机分为假手术组（5只），模型组（15只），低浓度怀牛膝加黄芪组（A组）15只，中浓度怀牛膝加黄芪组（B组）15只，高浓度怀牛膝加黄芪组（C组）15只，采用改良后Feency’s方法建立大鼠重型颅脑损伤模型。分别在1、3、7天3个时间点每组各取5只大鼠测定脑组织含水量，HE染色观察脑组织变化情况，并采用免疫组化方法检测脑组织AQP4的表达。结果模型组大鼠重型颅脑损伤后各时间点脑组织含水量、损伤灶周围AQP4的表达均高于假手术组（P＜0.05），HE染色观察发现模型组的脑组织肿胀水肿明显；A、B组各时间点脑组织含水量、AQP4表达水平与模型组相比较无明显降低（P＞0.05），HE染色观察发现与模型组基本一致；C组各时间点脑组织含水量、AQP4表达水平均较模型组降低（P＜0.05），HE染色观察发现与模型组比较，脑组织水肿情况有所改善。结论C组改善重型颅脑损伤后引起的脑水肿效果最明显，其作用机制可能与减少AQP4在损伤脑组织中的表达、减轻脑细胞损害有关。
목적：관찰저、중、고불동농도중약부우슬가황기전액대중형로뇌손상대서뇌조직함수량급수통도단백4（AQP4）표체적영향，탐토기치료중형뇌손상성뇌수종최가용약농도급궤제。방법장SD대서65지수궤분위가수술조（5지），모형조（15지），저농도부우슬가황기조（A조）15지，중농도부우슬가황기조（B조）15지，고농도부우슬가황기조（C조）15지，채용개량후Feency’s방법건립대서중형로뇌손상모형。분별재1、3、7천3개시간점매조각취5지대서측정뇌조직함수량，HE염색관찰뇌조직변화정황，병채용면역조화방법검측뇌조직AQP4적표체。결과모형조대서중형로뇌손상후각시간점뇌조직함수량、손상조주위AQP4적표체균고우가수술조（P＜0.05），HE염색관찰발현모형조적뇌조직종창수종명현；A、B조각시간점뇌조직함수량、AQP4표체수평여모형조상비교무명현강저（P＞0.05），HE염색관찰발현여모형조기본일치；C조각시간점뇌조직함수량、AQP4표체수평균교모형조강저（P＜0.05），HE염색관찰발현여모형조비교，뇌조직수종정황유소개선。결론C조개선중형로뇌손상후인기적뇌수종효과최명현，기작용궤제가능여감소AQP4재손상뇌조직중적표체、감경뇌세포손해유관。
Objective To observe the treatment effect of moisture content on brain organization and AQP 4 ex-pression of severe craniocerebral injury rats by using the traditional Chinese medicine achyranthes bidentata and as-tragalus membranaceus decoction in low ,middle and high pH indicator ;and to discuss the mechanism of curing se-vere craniocerebral injury brain edema . Methods Sixty five SD rats are divided randomly into sham-operation group(n=5) ,model group(n=15) ,low pH indicator achyranthes bidentata and astragalus membranaceus treated group(n=15) ,middle pH indicator treated group (n=15) ,and high pH indicator treated group (n=15) .Then to set up the severe brain injury model in rats by using improved Feency ’s methods .After that ,to get 5 rats in each group to measure the moisture content on brain organization ,to observe the changeable condition by using HE stai-ning ,and to detect the brain organization on AQP4 expression by using immunohistochemical ways on the first day , the third day and the seventh day . Results On each timing measure ,the moisture content on brain organization and the AQP4 expression around the injury part of the model group rats are higher than the sham-operation group (P<0.05) ,and the brain edema is obviously changed on the model group after using HE staining ;while the low and middle pH indicator achyranthes and astragalus membranaceus treated groups have no obvious reduction (P>0.05) ,and HE staining are almost the same as the model group .Then in the high pH indicator achyranthes bident-ata and astragalus membranaceus treated group ,the moisture content and the AQP4 expression are lower than the model group(P<0.05) ,and compared to the model group ,the brain edema has a improved change after using HE staining . Conclusion The brain edema caused by severe brain injury can be improved in the high pH indicator of achyranthes bidentata and astragalus membranaceus .Its mechanism of action may has a relation with the expression of AQP4 restraint in injury brain organization and the reduction of injury on brain cells .