新疆大学学报(自然科学版)
新疆大學學報(自然科學版)
신강대학학보(자연과학판)
XINJIANG UNIVERSITY JOURNAL(NATURAL SCIENCE EDITION)
2014年
2期
190-193
,共4页
王佳佳%吕小毅%李鹏%贾振红
王佳佳%呂小毅%李鵬%賈振紅
왕가가%려소의%리붕%가진홍
多孔硅%包虫病%微腔%生物传感器%反射光谱
多孔硅%包蟲病%微腔%生物傳感器%反射光譜
다공규%포충병%미강%생물전감기%반사광보
porous silicon%echinococcosis%microcavity%biosensor%reflectance spectrum
本文主要探讨制备用于包虫抗原DNA检测的n型多孔硅微腔生物传感器的可能性.高参杂n型硅片在HF酸/乙醇电解液中实现了具有良好性能的生物传感器基底的制备.通过检测多孔硅微腔浸入不同浓度的生物溶液前后的微腔反射谱的移动量来验证传感器的性能,溶液浓度从0.625μM至10.000μM,检测灵敏度为4.740 nm/μM.当微腔基底浸泡0.625μM生物溶液后,微腔红移5nm表明制备的生物传感器是成功的.根据浓度与微腔红移量之间的线性关系表明在多孔硅传感器上检测包虫病的可行性.
本文主要探討製備用于包蟲抗原DNA檢測的n型多孔硅微腔生物傳感器的可能性.高參雜n型硅片在HF痠/乙醇電解液中實現瞭具有良好性能的生物傳感器基底的製備.通過檢測多孔硅微腔浸入不同濃度的生物溶液前後的微腔反射譜的移動量來驗證傳感器的性能,溶液濃度從0.625μM至10.000μM,檢測靈敏度為4.740 nm/μM.噹微腔基底浸泡0.625μM生物溶液後,微腔紅移5nm錶明製備的生物傳感器是成功的.根據濃度與微腔紅移量之間的線性關繫錶明在多孔硅傳感器上檢測包蟲病的可行性.
본문주요탐토제비용우포충항원DNA검측적n형다공규미강생물전감기적가능성.고삼잡n형규편재HF산/을순전해액중실현료구유량호성능적생물전감기기저적제비.통과검측다공규미강침입불동농도적생물용액전후적미강반사보적이동량래험증전감기적성능,용액농도종0.625μM지10.000μM,검측령민도위4.740 nm/μM.당미강기저침포0.625μM생물용액후,미강홍이5nm표명제비적생물전감기시성공적.근거농도여미강홍이량지간적선성관계표명재다공규전감기상검측포충병적가행성.
The work in this paper has concentrated on fabrication possibility of biosensor for hydatid antigen DNA based on porous silicon microcavities. The substates have been fabricated from highly doped n-type silicon based on HF/ethanol electrolyte system. Examined the reflectance spectra before, after expore to different concentration of echinococcosis ranging from 0.625 μM to 10.000 μM, a detection sensitivity of 4.740 nm/μM has been demonstrated. A redshift of 5nm when the sample was exposed to 0.625 μM hydatid DNA solution. The linear relationship indicates that a potential and significate detection of echinococcosis.