CAJ | 학술논문

目的：体外研究法尼醇对白念珠菌生物被膜的抑制作用。方法：采用微量平板法制备12和24 h白念株菌生物被膜，每组膜分别加入不同浓度法尼醇（100～900μmol/L）培养24 h，甲基四氮盐（XTT）减低法检测法尼醇对白念珠菌生物被膜的抑制作用效果，倒置显微镜下观察生物被膜形态。结果：不同浓度的法尼醇对白念珠菌生物被膜均有抑制作用（P＜0．05），法尼醇浓度增加，抑制强度呈上升趋势。培养12 h，抑制白念株菌生物被膜50％活性的最低药物浓度（sessile minimal inhibitory concentration 50％，SMIC50）为600μmol/L；培养24 h，SMIC50为200μmol/L。结论：法尼醇对白念珠菌生物被膜生长具有明显抑制作用。法尼醇对白念珠菌生物被膜抑制强度与法尼醇浓度和生物被膜时相相关，高浓度法尼醇的抑制效果高于低浓度法尼醇。
목적：체외연구법니순대백념주균생물피막적억제작용。방법：채용미량평판법제비12화24 h백념주균생물피막，매조막분별가입불동농도법니순（100～900μmol/L）배양24 h，갑기사담염（XTT）감저법검측법니순대백념주균생물피막적억제작용효과，도치현미경하관찰생물피막형태。결과：불동농도적법니순대백념주균생물피막균유억제작용（P＜0．05），법니순농도증가，억제강도정상승추세。배양12 h，억제백념주균생물피막50％활성적최저약물농도（sessile minimal inhibitory concentration 50％，SMIC50）위600μmol/L；배양24 h，SMIC50위200μmol/L。결론：법니순대백념주균생물피막생장구유명현억제작용。법니순대백념주균생물피막억제강도여법니순농도화생물피막시상상관，고농도법니순적억제효과고우저농도법니순。
Objective:To evaluate the inhibitory activity of farnesol to the Candida albicans biofilms in vitro.Methods:Candida al-bicans biofilms were formed on flat-bottom 96-well microtiter plates and two study groups (12 h and 24 h Group)were noted,then re-spectively incubated in the RPMI 1640 with different concentration of farnesol (100-900 μmol/L)for 24 h.The XTT reduction assay was employed to evaluate the inhibitory effect of farnesol to the biofilms.Biofilm morphology was observed by inverted microscope.Re-sults:Farnesol (100-900 μmol/L)has inhibitory effect on Candida albicans biofilms.With the increase of concentration of farnesol,the inhibition rate tends to increas.The sessile minimal inhibitory concentration 50%(SMIC50 )of 12 h biofilm is 200 μmol/L;the SMIC50 of 24 h biofilm is 200 μmol/L.Conclusions:The inhibitory effect of Farnesol on Candida albicans biofilms was obvious.The inhibitory po-tency of farnesol was associated with its concentration and the phase of biofilms,and the farnesol of higher concentration are more effec-tive than that of lower concentration.