口腔生物医学
口腔生物醫學
구강생물의학
ORAL BIOMEDICINE
2014年
2期
75-77
,共3页
杨迷芳%杨天%王子露%王琰玲%吴友农
楊迷芳%楊天%王子露%王琰玲%吳友農
양미방%양천%왕자로%왕염령%오우농
中间普氏菌%牙龈成纤维细胞%核因子-κB受体活化因子配体%骨保护素
中間普氏菌%牙齦成纖維細胞%覈因子-κB受體活化因子配體%骨保護素
중간보씨균%아간성섬유세포%핵인자-κB수체활화인자배체%골보호소
Prevotella intermedia%Gingival fibroblasts%Receptor activator of NF-κB ligand%Osteoprotegerin
目的:探讨中间普氏菌培养上清对牙龈成纤维细胞核因子-κB受体活化因子配体(receptor activator for nuclear factor-κB ligand,RANKL)、骨保护素(osteoprotegerin,OPG)表达的影响。方法:将系列浓度的中间普氏菌培养上清作用于牙龈成纤维细胞,显微镜下观察细胞的变化。实时定量 RT-PCR法和 Western blot法分别检测 RANKL、OPG mRNA及蛋白水平的表达,并计算 RANKL/OPG的比值。结果:牙龈成纤维细胞的数量随中间普氏菌培养上清浓度的增加而减少,50μg/mL 时,显微镜下没有活细胞存在。在mRNA水平上和蛋白水平上,RANKL/OPG的比值在12.5μg/mL处理组均高于对照组,差异具有统计学意义(P<0.01)。结论:中间普氏菌培养上清可诱导人牙龈成纤维细胞表达 RANKL 和 OPG,破坏 RANKL/OPG比例的平衡,影响破骨细胞分化的细胞因子微环境,在破骨细胞分化和牙周炎骨吸收中起着一定的调节作用。
目的:探討中間普氏菌培養上清對牙齦成纖維細胞覈因子-κB受體活化因子配體(receptor activator for nuclear factor-κB ligand,RANKL)、骨保護素(osteoprotegerin,OPG)錶達的影響。方法:將繫列濃度的中間普氏菌培養上清作用于牙齦成纖維細胞,顯微鏡下觀察細胞的變化。實時定量 RT-PCR法和 Western blot法分彆檢測 RANKL、OPG mRNA及蛋白水平的錶達,併計算 RANKL/OPG的比值。結果:牙齦成纖維細胞的數量隨中間普氏菌培養上清濃度的增加而減少,50μg/mL 時,顯微鏡下沒有活細胞存在。在mRNA水平上和蛋白水平上,RANKL/OPG的比值在12.5μg/mL處理組均高于對照組,差異具有統計學意義(P<0.01)。結論:中間普氏菌培養上清可誘導人牙齦成纖維細胞錶達 RANKL 和 OPG,破壞 RANKL/OPG比例的平衡,影響破骨細胞分化的細胞因子微環境,在破骨細胞分化和牙週炎骨吸收中起著一定的調節作用。
목적:탐토중간보씨균배양상청대아간성섬유세포핵인자-κB수체활화인자배체(receptor activator for nuclear factor-κB ligand,RANKL)、골보호소(osteoprotegerin,OPG)표체적영향。방법:장계렬농도적중간보씨균배양상청작용우아간성섬유세포,현미경하관찰세포적변화。실시정량 RT-PCR법화 Western blot법분별검측 RANKL、OPG mRNA급단백수평적표체,병계산 RANKL/OPG적비치。결과:아간성섬유세포적수량수중간보씨균배양상청농도적증가이감소,50μg/mL 시,현미경하몰유활세포존재。재mRNA수평상화단백수평상,RANKL/OPG적비치재12.5μg/mL처리조균고우대조조,차이구유통계학의의(P<0.01)。결론:중간보씨균배양상청가유도인아간성섬유세포표체 RANKL 화 OPG,파배 RANKL/OPG비례적평형,영향파골세포분화적세포인자미배경,재파골세포분화화아주염골흡수중기착일정적조절작용。
Objective:To evaluate the effects of Prevotella intermedia supernatant on the expression of receptor activator of NF-κB ligand (RANKL)and osteoprotegerin (OPG)in human gingival fibroblasts (HGFs).Methods:The HGFs were treated with gradient Prevotella intermedia supernatant.HGFs were observed under the microscope.The real-time RT-PCR and Western blot analysis were used for testing the expression of RANKL and OPG,and the RANKL/OPG ratio was calculated.Results:The number of cells decreased with increase of the supernatant concentration.At 50 μg/mL,no cell survival could be observed under the microscope.The ratio of RANKL/OPG was increased in the cells treated with 12.5 μg/mL of Prevotella intermedia supernatant(P<0.01).Conclusions:Prevo-tella intermedia supernatant could disrupt the homeostasis of RANKL/OPG,destroy the microenvironment of osteoclast differentiation. Prevotella intermedia supernatant may play a regulatory role in osteoclast differentiation and bone resorption in periodontitis.