中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2013年
11期
1179-1181
,共3页
李皓雲%黄震浩%黄婧娟%张琳琳%段俊丽
李皓雲%黃震浩%黃婧娟%張琳琳%段俊麗
리호운%황진호%황청연%장림림%단준려
新生血管化%缺血%原花青素%低氧诱导因子-1%血管内皮生长因子
新生血管化%缺血%原花青素%低氧誘導因子-1%血管內皮生長因子
신생혈관화%결혈%원화청소%저양유도인자-1%혈관내피생장인자
Neovascularization%Ischemia%Procyanidins%Hypoxia inducible factor-1%Vascular endothelial growth factor
目的 探讨原花青素对大鼠下肢缺血后血管新生的影响及其相关作用机制.方法 将SD雄性大鼠12只随机分为两组:原花青素组和对照组,每组各6只；建立下肢缺血模型,两组每日均灌胃给药,原花青素组给予原花青素200 mg/(kg·d),对照组给予同体积生理盐水.于术后第14天取大鼠缺血下肢的下肢内收肌组织,用免疫组织化学法检测CD31在缺血肌肉组织中的表达及新生血管数量,用蛋白印迹法检测低氧诱导因子-1蛋白表达,用酶联免疫吸附试验法测定血管内皮生长因子含量.结果 原花青素组缺血肌肉组织中毛细血管计数(169.67±3.11)个/HP明显高于对照组(111.00±3.11)个/HP),差异有统计学意义(t=13.350,P＜0.0001]；原花青素组低氧诱导因子-1蛋白表达(1.90±0.25)明显高于对照组(0.54±0.21),差异也有统计学意义(t=4.183,P=0.0058]；血管内皮生长因子含量(432.86±13.00) μg/L也明显高于对照组(326.68±11.08) μg/L,差异有统计学意义(t=6.216,P＜0.0001].结论 原花青素对缺血后血管新生有促进作用,这一作用可能与原花青素诱导低氧诱导因子-1蛋白表达上调,继而使血管内皮生长因子增加,从而通过多条信号通路诱导新生血管生成增多有关.
目的 探討原花青素對大鼠下肢缺血後血管新生的影響及其相關作用機製.方法 將SD雄性大鼠12隻隨機分為兩組:原花青素組和對照組,每組各6隻；建立下肢缺血模型,兩組每日均灌胃給藥,原花青素組給予原花青素200 mg/(kg·d),對照組給予同體積生理鹽水.于術後第14天取大鼠缺血下肢的下肢內收肌組織,用免疫組織化學法檢測CD31在缺血肌肉組織中的錶達及新生血管數量,用蛋白印跡法檢測低氧誘導因子-1蛋白錶達,用酶聯免疫吸附試驗法測定血管內皮生長因子含量.結果 原花青素組缺血肌肉組織中毛細血管計數(169.67±3.11)箇/HP明顯高于對照組(111.00±3.11)箇/HP),差異有統計學意義(t=13.350,P＜0.0001]；原花青素組低氧誘導因子-1蛋白錶達(1.90±0.25)明顯高于對照組(0.54±0.21),差異也有統計學意義(t=4.183,P=0.0058]；血管內皮生長因子含量(432.86±13.00) μg/L也明顯高于對照組(326.68±11.08) μg/L,差異有統計學意義(t=6.216,P＜0.0001].結論 原花青素對缺血後血管新生有促進作用,這一作用可能與原花青素誘導低氧誘導因子-1蛋白錶達上調,繼而使血管內皮生長因子增加,從而通過多條信號通路誘導新生血管生成增多有關.
목적 탐토원화청소대대서하지결혈후혈관신생적영향급기상관작용궤제.방법 장SD웅성대서12지수궤분위량조:원화청소조화대조조,매조각6지；건립하지결혈모형,량조매일균관위급약,원화청소조급여원화청소200 mg/(kg·d),대조조급여동체적생리염수.우술후제14천취대서결혈하지적하지내수기조직,용면역조직화학법검측CD31재결혈기육조직중적표체급신생혈관수량,용단백인적법검측저양유도인자-1단백표체,용매련면역흡부시험법측정혈관내피생장인자함량.결과 원화청소조결혈기육조직중모세혈관계수(169.67±3.11)개/HP명현고우대조조(111.00±3.11)개/HP),차이유통계학의의(t=13.350,P＜0.0001]；원화청소조저양유도인자-1단백표체(1.90±0.25)명현고우대조조(0.54±0.21),차이야유통계학의의(t=4.183,P=0.0058]；혈관내피생장인자함량(432.86±13.00) μg/L야명현고우대조조(326.68±11.08) μg/L,차이유통계학의의(t=6.216,P＜0.0001].결론 원화청소대결혈후혈관신생유촉진작용,저일작용가능여원화청소유도저양유도인자-1단백표체상조,계이사혈관내피생장인자증가,종이통과다조신호통로유도신생혈관생성증다유관.
Objective To investigate the effect of proanthcyaindin on angiogenesis of rats with ischemic hindlimb.Methods Twelve male SD rats were randomized divided into control group (n =6) and proanthcyaindin group (PC group,n =6).Lower limb ischemia rat model was establish,rats in both group were administration by oral daily,and PC group was given proanthocyanidins 200 mg/(kg · d),while the control group received the same volume of saline.Forteen days after surgery,ischemic tissues of adductor were collected for several tests including the expression of CD31 in the ischemic muscle tissue and the number of new blood vessels by immunohistochemical staining,hypoxia inducible factor-1 protein expression by western blot,and vascular endothelial growth factor level test by enzyme-linked immunosorbent assay.Results The capillary count showed that the angiogenesis situation of PC group was (69.67 ±3.11)/HP,higher than that in control group((111.00 ± 3.11)/HP,t =13.350,P ＜ 0.0001).The HIF-1 protein expression in PC group was (1.90 ± 0.25),remarkable higher than that in control group (0.54 ± 0.21,t =4.183,P =0.0058).Compared with control group,VEGF level in PC group increased((432.86 ± 13.00) μg/L vs.(326.68 ± 11.08) μg/L,t =6.216,P ＜0.0001).Conclusion Proanthcyaindin plays a positive role in angiogenesis after ischemia.PC may induce up-regulation of HIF-1 and VEGF,and then promote the formation of endovascular through multiple signaling pathways consequently.
