CAJ | 학술논문

目的：评价丹曲林预先给药对脓毒症大鼠膈肌功能的影响。方法雄性SD大鼠30只，体重200～220 g ，9～10周龄。采用随机数字表法，将大鼠分为3组（ n＝10）：假手术组（S组）、脓毒症组（CLP组）和丹曲林组（D组）。D组腹腔注射丹曲林6 mg/kg （用二甲基亚砜稀释至500μl ）。1 h后CLP组和D组采用盲肠结扎穿孔法制备脓毒症模型。于术后24 h时取左、右膈肌组织，左膈肌测定收缩功能[单刺激肌颤搐、最大收缩期上升速率（dmax/dt ）、最大舒张期下降速率（dmin/dt ）、最大强直收缩力、张力-频率曲线和疲劳指数]。结果与S组比较，CLP组膈肌单刺激肌颤搐、dmax/dt和dmin/dt、最大强直收缩力、疲劳指数均降低，D组单刺激肌颤搐、单刺激dmax/dt和dmin/dt、最大强直收缩力降低，疲劳指数升高，CLP组和D组张力-频率曲线下移（ P＜0.05或0.01）；与CLP组比较，D组膈肌单刺激肌颤搐、dmax/dt和dmin/dt、最大强直收缩力和疲劳指数均升高（ P＜0.05或0.01）。结论丹曲林预先给药可改善脓毒症大鼠膈肌功能。
목적：평개단곡림예선급약대농독증대서격기공능적영향。방법웅성SD대서30지，체중200～220 g ，9～10주령。채용수궤수자표법，장대서분위3조（ n＝10）：가수술조（S조）、농독증조（CLP조）화단곡림조（D조）。D조복강주사단곡림6 mg/kg （용이갑기아풍희석지500μl ）。1 h후CLP조화D조채용맹장결찰천공법제비농독증모형。우술후24 h시취좌、우격기조직，좌격기측정수축공능[단자격기전휵、최대수축기상승속솔（dmax/dt ）、최대서장기하강속솔（dmin/dt ）、최대강직수축력、장력-빈솔곡선화피로지수]。결과여S조비교，CLP조격기단자격기전휵、dmax/dt화dmin/dt、최대강직수축력、피로지수균강저，D조단자격기전휵、단자격dmax/dt화dmin/dt、최대강직수축력강저，피로지수승고，CLP조화D조장력-빈솔곡선하이（ P＜0.05혹0.01）；여CLP조비교，D조격기단자격기전휵、dmax/dt화dmin/dt、최대강직수축력화피로지수균승고（ P＜0.05혹0.01）。결론단곡림예선급약가개선농독증대서격기공능。
Objective To evaluate the effects of dantrolene pretreatment on diaphragmatic function in septic rats .Methods Thirty adult male Sprague-Dawley rats , weighing 200-220 g , aged 9-10 weeks , were randomized into 3 groups (n=10 each) using a random number table :sham operation group (group S) ,spesis group (group CLP) and dantrolene group (group D) .The animals were anesthetized with pentobarbital sodium . Dantrolene 6 mg/kg was injected intraperitoneally (in dimethyl sulfoxide 500 μl ) .Sepsis was induced by cecal ligation and puncture 1 h later in CLP and D groups .The left and right diaphragm was rapidly excised at 24 h after cecal ligation and puncture . The left diaphragm was used to detect the systolic function including the single stimulation twitch , dmax/dt , dmin/dt , maximal force of tetanic contraction , force-frequency curves , and fatigue index .Results Compared with group S ,the single twitch myopalmus ,dmax/dt ,dmin/dt ,maximum peak tension and fatigue index were significantly decreased in group CLP ,the single stimulation twitch ,dmax/dt ,dmin/dt and maximal force of tetanic contraction were decreased , fatigue index was increased in group D , and the force-frequency curve was shifted downward in CLP and D groups ( P<0.05 or 0.01) .Compared with group CLP ,the single stimulation twitch ,dmax/dt ,dmin/dt ,maximal force of tetanic contraction and fatigue index were significantly increased in group D ( P< 0.05 or 0.01 ) .Conclusion Dantrolene pretreatment can improve diaphragmatic function in septic rats .