中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
3期
293-296
,共4页
张亮%闵苏%黎平%律峰%郝学超%谢飞%陈其彬%刘力%沈一维%朱贤琳%魏珂%陈婧
張亮%閔囌%黎平%律峰%郝學超%謝飛%陳其彬%劉力%瀋一維%硃賢琳%魏珂%陳婧
장량%민소%려평%률봉%학학초%사비%진기빈%류력%침일유%주현림%위가%진청
胰高血糖素样肽1%认知障碍%海马%炎症%血糖
胰高血糖素樣肽1%認知障礙%海馬%炎癥%血糖
이고혈당소양태1%인지장애%해마%염증%혈당
Glucagon-like peptide 1%Cognition disorders%Hippocampus%Inflammation%Blood glucose
目的：评价艾塞那肽对老龄大鼠海马星型胶质酸性蛋白（GFAP ）和白介素-1β（IL-1β）表达的影响。方法清洁级健康雄性SD大鼠48只，22～24周龄，体重500～700 g ，采用随机数字表法分为4组（ n＝12）：对照组（C组）、艾塞那肽组（E组）、手术组（O组）和艾塞那肽＋手术组（OE组）。C组和E组未实施麻醉及手术；O组和OE组行脾切除术；OE组于切皮前30 min和术后12 h时、E组于相应时点腹腔注射艾塞那肽5μg/kg ，C组和O组给予等容量生理盐水。于术前1 d （T0）时采用Morris水迷宫实验测定逃避潜伏期（EL ）和游泳总路程（TSD ）评价学习记忆功能，于麻醉后（T1）、术毕即刻（T2）及术后1 d （T3）时采集尾静脉血，测定空腹血糖；于术后1 d测定认知功能后处死大鼠取海马，采用免疫组化法检测GFAP表达，Western blot法检测IL-1β表达。结果4组大鼠T0时EL和TSD比较差异无统计学意义（ P＞0.05）。与C组比较，O组和OE组T3时EL、TSD和T2，3时血糖升高，T3时海马GFAP和IL-1β表达上调（ P＜0.05）；与O组比较，OE组T3时EL、TSD和T2，3时血糖降低，T3时海马GFAP和IL-1β表达下调（ P＜0.05）。结论艾塞那肽通过抑制海马炎性反应和稳定围术期血糖改善老龄大鼠术后认知功能。
目的：評價艾塞那肽對老齡大鼠海馬星型膠質痠性蛋白（GFAP ）和白介素-1β（IL-1β）錶達的影響。方法清潔級健康雄性SD大鼠48隻，22～24週齡，體重500～700 g ，採用隨機數字錶法分為4組（ n＝12）：對照組（C組）、艾塞那肽組（E組）、手術組（O組）和艾塞那肽＋手術組（OE組）。C組和E組未實施痳醉及手術；O組和OE組行脾切除術；OE組于切皮前30 min和術後12 h時、E組于相應時點腹腔註射艾塞那肽5μg/kg ，C組和O組給予等容量生理鹽水。于術前1 d （T0）時採用Morris水迷宮實驗測定逃避潛伏期（EL ）和遊泳總路程（TSD ）評價學習記憶功能，于痳醉後（T1）、術畢即刻（T2）及術後1 d （T3）時採集尾靜脈血，測定空腹血糖；于術後1 d測定認知功能後處死大鼠取海馬，採用免疫組化法檢測GFAP錶達，Western blot法檢測IL-1β錶達。結果4組大鼠T0時EL和TSD比較差異無統計學意義（ P＞0.05）。與C組比較，O組和OE組T3時EL、TSD和T2，3時血糖升高，T3時海馬GFAP和IL-1β錶達上調（ P＜0.05）；與O組比較，OE組T3時EL、TSD和T2，3時血糖降低，T3時海馬GFAP和IL-1β錶達下調（ P＜0.05）。結論艾塞那肽通過抑製海馬炎性反應和穩定圍術期血糖改善老齡大鼠術後認知功能。
목적：평개애새나태대노령대서해마성형효질산성단백（GFAP ）화백개소-1β（IL-1β）표체적영향。방법청길급건강웅성SD대서48지，22～24주령，체중500～700 g ，채용수궤수자표법분위4조（ n＝12）：대조조（C조）、애새나태조（E조）、수술조（O조）화애새나태＋수술조（OE조）。C조화E조미실시마취급수술；O조화OE조행비절제술；OE조우절피전30 min화술후12 h시、E조우상응시점복강주사애새나태5μg/kg ，C조화O조급여등용량생리염수。우술전1 d （T0）시채용Morris수미궁실험측정도피잠복기（EL ）화유영총로정（TSD ）평개학습기억공능，우마취후（T1）、술필즉각（T2）급술후1 d （T3）시채집미정맥혈，측정공복혈당；우술후1 d측정인지공능후처사대서취해마，채용면역조화법검측GFAP표체，Western blot법검측IL-1β표체。결과4조대서T0시EL화TSD비교차이무통계학의의（ P＞0.05）。여C조비교，O조화OE조T3시EL、TSD화T2，3시혈당승고，T3시해마GFAP화IL-1β표체상조（ P＜0.05）；여O조비교，OE조T3시EL、TSD화T2，3시혈당강저，T3시해마GFAP화IL-1β표체하조（ P＜0.05）。결론애새나태통과억제해마염성반응화은정위술기혈당개선노령대서술후인지공능。
Objective To evaluate the effects of exendin-4 on glial brillary acidic protein (GFAP ) and interleukin-1β(IL-1β) expression in hippocampi of aged rats .Methods Forty-eight healthy male Sprague-Dawley rats ,aged 22-24 weeks ,weighing 500-700 g ,were randomly divided into 4 groups (n=12 each) using a random number table:control group (group C ) ,exendin-4 group (group E ) ,operation group (group O ) and exendin-4 plus operation group (group OE) .The rats were anesthetized with intraperitoneal fentanyl and droperidol .Groups C and E did not receive anesthesia or splenectomy .In O and OE groups ,splenectomy was carried out .In E and OE groups , exendin-4 5 μg/kg was injected intraperitoneally at 30 min before skin incision and 12 h after operation .C and O groups received the equal volume of normal saline instead of exendin-4 .Learning and memory function was assessed using Morris water maze test (escape latency (EL) and total swimming distance (TSD) at 1 day before operation (T0 ) .The fasting blood glucose was measured after anesthesia (T1 ) ,at the end of operation (T2 ) and on postoperative day 1 (T3 ) .The rats were sacrificed after assessment of the cognitive function at T 3 and the hippocampi were removed for determination of the expression of GFAP (by immuno-histochemistry ) and IL-1β(by Western blot ) .Results There was no significant difference in the EL and TSD at T0 between the four groups ( P>0.05) .Compared with group C ,the EL and TSD were significantly prolonged at T3 and fasting blood glucose was increased at T2 ,3 ,and the expression of IL-1βand GFAP was up-regulated at T3 in O and OE groups ( P<0.05) .Compared with group O ,the EL and TSD were significantly prolonged at T3 and fasting blood glucose was decreased at T2 ,3 ,and the expression of IL-1βand GFAP was down-regulated at T3 in group OE ( P<0.05) . Conclusion Exendin-4 can improve the postoperative cognitive function of aged rats by inhibiting inflammatory responses in hippocampi and maintaining stable perioperative blood glucose .
