CAJ | 학술논문

目的：探讨脊髓神经元限制性沉默因子（NRSF ）在瑞芬太尼诱发切口痛小鼠痛觉过敏中的作用。方法健康清洁级成年雄性昆明小鼠56只，体重20～25 g ，采用随机数字表法分为7组（ n＝8）：对照组（C组）、切口痛组（I组）、切口痛＋瑞芬太尼组（IR组）、NRSF反义寡核苷酸组（NAS组）、切口痛＋NRSF反义寡核苷酸组（I＋NAS组）、切口痛＋瑞芬太尼＋NRSF错义寡核苷酸（IR＋NMS组）和切口痛＋瑞芬太尼＋NRSF反义寡核苷酸（IR＋NAS组）。C组、I组和IR组鞘内注射人工脑脊液5μl ，NAS组、I＋NAS组和IR＋NAS组鞘内注射NRSF反义寡核苷酸10μg ，IR＋ NMS组鞘内注射NRSF错义寡核苷酸10μg ，均为1次/d ，连续3 d。于末次鞘内注射后30 min时，C组和NAS组皮下输注生理盐水0.4 ml ，I组和I＋NAS组制备切口痛模型，同时皮下输注生理盐水0.4 ml ，IR组、IR＋NMS组和IR＋NAS组制备切口痛模型，同时皮下输注瑞芬太尼0.04 mg/kg。于术前3 d （T0）、4 h （T1）、术后4 h（T2）、12 h（T3）、24 h（T4）和48 h（T5）时测定机械缩足阈（PWMT）和热缩足阈（PWTL）。结果与C组比较，I组、IR组、I＋NAS组、IR＋NMS组和IR＋NAS组T2-5时PWMT和PWTL降低（ P＜0.05），NAS组各时点PWMT和PWTL差异无统计学意义（ P＞0.05）；与I组比较，IR组和IR＋NMS组T2-5时PWMT和PWTL降低（ P＜0.05），I＋NAS组各时点PWMT和PWTL差异无统计学意义（ P＞0.05）；与IR组比较， IR＋NMS组各时点PWMT和PWTL差异无统计学意义（ P＞0.05），IR＋NAS组T2-5时PWMT和PWTL升高（ P＜0.05）。结论脊髓NRSF参与了瑞芬太尼诱发切口痛小鼠痛觉过敏的形成和维持。
목적：탐토척수신경원한제성침묵인자（NRSF ）재서분태니유발절구통소서통각과민중적작용。방법건강청길급성년웅성곤명소서56지，체중20～25 g ，채용수궤수자표법분위7조（ n＝8）：대조조（C조）、절구통조（I조）、절구통＋서분태니조（IR조）、NRSF반의과핵감산조（NAS조）、절구통＋NRSF반의과핵감산조（I＋NAS조）、절구통＋서분태니＋NRSF착의과핵감산（IR＋NMS조）화절구통＋서분태니＋NRSF반의과핵감산（IR＋NAS조）。C조、I조화IR조초내주사인공뇌척액5μl ，NAS조、I＋NAS조화IR＋NAS조초내주사NRSF반의과핵감산10μg ，IR＋ NMS조초내주사NRSF착의과핵감산10μg ，균위1차/d ，련속3 d。우말차초내주사후30 min시，C조화NAS조피하수주생리염수0.4 ml ，I조화I＋NAS조제비절구통모형，동시피하수주생리염수0.4 ml ，IR조、IR＋NMS조화IR＋NAS조제비절구통모형，동시피하수주서분태니0.04 mg/kg。우술전3 d （T0）、4 h （T1）、술후4 h（T2）、12 h（T3）、24 h（T4）화48 h（T5）시측정궤계축족역（PWMT）화열축족역（PWTL）。결과여C조비교，I조、IR조、I＋NAS조、IR＋NMS조화IR＋NAS조T2-5시PWMT화PWTL강저（ P＜0.05），NAS조각시점PWMT화PWTL차이무통계학의의（ P＞0.05）；여I조비교，IR조화IR＋NMS조T2-5시PWMT화PWTL강저（ P＜0.05），I＋NAS조각시점PWMT화PWTL차이무통계학의의（ P＞0.05）；여IR조비교， IR＋NMS조각시점PWMT화PWTL차이무통계학의의（ P＞0.05），IR＋NAS조T2-5시PWMT화PWTL승고（ P＜0.05）。결론척수NRSF삼여료서분태니유발절구통소서통각과민적형성화유지。
Objective To evaluate the role of neuron-restrictive silencer factor (NRSF) in the spinal cord in remifentanil-induced hyperalgesia in a mouse model of incisional pain (IP) .Methods Fifty-six male Kunming mice were randomly divided into 7 groups (n=8 each):control group (group C) ,IP group (group I) ,IP +remifentanil group (group IR ) , NRSF antisense oligonucleotide group (NAS group ) , IP + NRSF antisense oligonucleotide group (I+NAS group ) ,IP + remifentanil + NRSF mismatch oligonucleotide group (IR+NMS group) , and IP + remifentanil + NRSF antisense oligonucleotide group (IR + NAS group ) . Artificial cerebrospinal fluid 5 μl was injected intrathecally once a day for 3 consecutive days in C ,I and IR groups .NRSF antisense oligonucleotide NAS 10μg was injected intrathecally once a day for 3 consecutive days in NAS ,I+NAS and IR + NAS groups . NRSF mismatch oligonucleotide 10 μg was injected intrathecally once a day for 3 consecutive days in IR+NMS group .A 1-cm longitudinal incision was made through skin ,fascia and muscle of the plantar aspect of the right hindpaw to establish the model of incisional pain in sevoflurane-anesthetized rats .At 30 min after the last injection ,normal saline 0.4 ml was infused subcutaneously in C and NAS groups ,the model was established and normal saline 0.4 ml was subcutaneously infused simultaneously in I and I+NAS groups ,and the model was established and remifentanil 0.04 mg/kg was subcutaneously infused simultaneously in IR ,IR+NMS and IR+NAS groups .At 3 days before operation (T0 ) ,4 h before operation (T1 ) and 4 ,12 ,24 and 48 h after operation (T1-5 ) ,mechanical paw withdrawal threshold to von Frey stimuli (PMWT ) and paw withdrawal latency to thermal nociceptive stimulus (PTWL ) were measured .Results Compared with C group ,the PWMT and PWTL were significantly decreased at T2-5 in I ,IR ,I+NAS ,IR+NMS and IR+NAS groups ( P<0.05) ,and no significant change was found in the PWMT and PWTL at each time point in NAS group ( P>0.05 ) .Compared with I group ,the PWMT and PWTL were significantly decreased at T2-5 in IR and IR+NMS groups ( P<0.05) , and no significant change was found in the PWMT and PWTL at each time point in I +NAS group ( P>0.05) . Compared with IR group ,no significant change was found in the PWMT and PWTL at each time point in IR+NMS group ( P>0.05) ,and the PWMT and PWTL were significantly increased at T2-5 in IR+NAS group ( P<0.05) . Conclusion NRSF in the spinal cord is involved in the development and maintenance of hyperalgesia induced by remifentanil in a mouse model of IP .