CAJ | 학술논문

目的：评价脂联素对脓毒症大鼠肺损伤时线粒体损伤的影响。方法清洁级雄性Wistar大鼠60只，7～8周龄，体重180～220 g ，采用随机数字表法分为3组（ n＝20）：假手术组（S组）、脓毒症组（SEP组）和脂联素组（APN组）。采用盲肠结扎穿孔（CLP ）法制备脓毒症大鼠肺损伤模型。S组剖腹后仅分离盲肠远端，不结扎穿孔，术毕关腹。APN组CLP后12 h时腹腔注射基因重组脂联素6 mg/kg。CLP后24 h时每组随机取10只大鼠处死取肺，采用ELISA法测定IL-6和高迁移率族蛋白B1（HMGB1）含量；提取肺组织线粒体，采用硫代巴比妥酸比色法测定肺组织线粒体MDA含量，分光光度法测定线粒体膜肿胀程度和线粒体活力。术后7 d观察每组余10只大鼠的生存情况。结果与S组比较，SEP组肺组织IL-6和HMGB1含量、线粒体MDA含量升高，线粒体膜肿胀程度增加，线粒体活力降低，术后7 d生存率降低（ P＜0.05）；与SEP组比较，APN组肺组织IL-6和HMGB1含量、线粒体MDA含量降低，线粒体膜肿胀程度减轻，线粒体活力升高，术后7 d生存率升高（ P＜0.05）。结论脂联素对脓毒症大鼠肺保护作用的机制与减轻肺组织线粒体损伤有关。
목적：평개지련소대농독증대서폐손상시선립체손상적영향。방법청길급웅성Wistar대서60지，7～8주령，체중180～220 g ，채용수궤수자표법분위3조（ n＝20）：가수술조（S조）、농독증조（SEP조）화지련소조（APN조）。채용맹장결찰천공（CLP ）법제비농독증대서폐손상모형。S조부복후부분리맹장원단，불결찰천공，술필관복。APN조CLP후12 h시복강주사기인중조지련소6 mg/kg。CLP후24 h시매조수궤취10지대서처사취폐，채용ELISA법측정IL-6화고천이솔족단백B1（HMGB1）함량；제취폐조직선립체，채용류대파비타산비색법측정폐조직선립체MDA함량，분광광도법측정선립체막종창정도화선립체활력。술후7 d관찰매조여10지대서적생존정황。결과여S조비교，SEP조폐조직IL-6화HMGB1함량、선립체MDA함량승고，선립체막종창정도증가，선립체활력강저，술후7 d생존솔강저（ P＜0.05）；여SEP조비교，APN조폐조직IL-6화HMGB1함량、선립체MDA함량강저，선립체막종창정도감경，선립체활력승고，술후7 d생존솔승고（ P＜0.05）。결론지련소대농독증대서폐보호작용적궤제여감경폐조직선립체손상유관。
Objective To evaluate the effects of adiponectin on the mitochondrial damage in septic rats with lung injury .Methods Sixty male Wistar rats ,aged 7-8 weeks ,weighing 180-220 g ,were randomly divided into 3 groups (n=20 each) using a random number table :sham operation group (S group) ,sepsis group (SEP group) and adiponectin group (APN group) .The animals were anesthetized with intraperitoneal 2% pentobarbital sodium 50 mg/kg .Sepsis was produced by cecum ligation and puncture (CLP) .In APN groups ,gene recombinant adiponectin 6 mg/kg was injected intraperitoneally at 12 h after CLP .At 24 h after the operation ,10 rats from each group were chosen and sacrificed ,and lungs were removed for detection of interleukin-6 (IL-6 ) and high-mobility group box-1 (HMGB1 ) contents . The mitochondria of lung samples were isolated for measurement of the malondialdehyde (MDA ) content and degree of mitochondrial swelling and mitochondrial activity . The survival rates within 7 days after operation were recorded in the left 10 rats in each group .Results Compared with S group , IL-6 and HMGB1 contents in lung tissues and MDA content in the mitochondria were significantly increased ,the degree of mitochondrial swelling was increased , and the mitochondrial activity and survival rates within 7 days after operation were decreased in SEP group ( P<0.05 ) . Compared with SEP group , IL-6 and HMGB1 contents in lung tissues and MDA content in the mitochondria were significantly decreased ,the degree of mitochondrial swelling was reduced ,and the mitochondrial activity and survival rates within 7 days after operation were increased in APN groups ( P< 0.05 ) .Conclusion Adiponectin can attenuate lung injury and raise the survival rates in septic rats ,and reduction of mitochondrial damage in lung tissues is involved in the mechanism .