CAJ | 학술논문

目的：评价肾缺血再灌注损伤小鼠肾组织调节性T淋巴细胞（Treg细胞）趋化因子受体3（CXCR3）表达的变化。方法雄性SPF级C57BL/6小鼠48只，8～12周龄，体重20～25 g ，采用随机数字表法，将其分为3组（ n＝16）：假手术组（S组）、肾缺血再灌注组（I/R组）和CD25单克隆抗体PC61组（P组）。采用阻断双侧肾蒂45 min再灌注的方法制备肾缺血再灌注损伤模型。P组于模型制备前24 h时腹腔注射PC61250μg。I/R组和P组于再灌注24 h（T1）和72 h（T2）时、S组于相应时点取下腔静脉血样，测定血清BUN和Cr的浓度，随后取双肾，进行肾组织损伤评分，测定CD4＋ CD25＋ Foxp3＋Treg细胞和CXCR3＋CD4＋CD25＋ Foxp3＋Treg细胞的数量。结果与S组比较，I/R组和P组T1，2时血清BUN、Cr的浓度和肾组织损伤评分升高，I/R组T2时CD4＋CD25＋ Foxp3＋Treg细胞和CXCR3＋CD4＋CD25＋Foxp3＋Treg细胞的数量增多（ P＜0.05）；与I/R组比较，P组T2时血清BUN、Cr浓度和肾组织损伤评分升高，CD4＋ CD25＋ Foxp3＋ Treg细胞和CXCR3＋ CD4＋ CD25＋ Foxp3＋ Treg细胞的数量减少（ P＜0.05）。结论 CXCR3表达上调有助于Treg细胞迁移至肾缺血再灌注损伤小鼠的肾组织。
목적：평개신결혈재관주손상소서신조직조절성T림파세포（Treg세포）추화인자수체3（CXCR3）표체적변화。방법웅성SPF급C57BL/6소서48지，8～12주령，체중20～25 g ，채용수궤수자표법，장기분위3조（ n＝16）：가수술조（S조）、신결혈재관주조（I/R조）화CD25단극륭항체PC61조（P조）。채용조단쌍측신체45 min재관주적방법제비신결혈재관주손상모형。P조우모형제비전24 h시복강주사PC61250μg。I/R조화P조우재관주24 h（T1）화72 h（T2）시、S조우상응시점취하강정맥혈양，측정혈청BUN화Cr적농도，수후취쌍신，진행신조직손상평분，측정CD4＋ CD25＋ Foxp3＋Treg세포화CXCR3＋CD4＋CD25＋ Foxp3＋Treg세포적수량。결과여S조비교，I/R조화P조T1，2시혈청BUN、Cr적농도화신조직손상평분승고，I/R조T2시CD4＋CD25＋ Foxp3＋Treg세포화CXCR3＋CD4＋CD25＋Foxp3＋Treg세포적수량증다（ P＜0.05）；여I/R조비교，P조T2시혈청BUN、Cr농도화신조직손상평분승고，CD4＋ CD25＋ Foxp3＋ Treg세포화CXCR3＋ CD4＋ CD25＋ Foxp3＋ Treg세포적수량감소（ P＜0.05）。결론 CXCR3표체상조유조우Treg세포천이지신결혈재관주손상소서적신조직。
Objective To evaluate the changes in the expression of CXCR3 in regulatory T cells (Tregs) in the renal tissues of mice with renal ischemia-reperfusion (I/R) injury .Methods Forty-eight SPF male C57BL/6J mice ,aged 8-12 yr ,weighing 20-25 g ,were randomly divided into 3 groups ( n=16 each ) using a random number table:sham operation group (group S) ,group I/R and CD25 monoclonal antibody PC61 group (group P) . Bilateral kidneys were exposed and their pedicles were occluded for 45 min with atraumatic mini-clamp followed by 72 h reperfusion .PC61 250 μg was injected intraperitoneally at 24 h before the model was established .Blood samples were collected from the inferior vena cava at 24 and 72 h of reperfusion (T1 ,2 ) for determination of serum blood urea nitrogen (BUN) and creatinine (Cr) concentrations .Bilateral kidneys were obtained for determination of CD4+ CD25+ Foxp3+ Treg count and CXCR3+ CD4+ CD25+ Foxp3+ Treg count in renal tissues and the pathological changes of the kidney were scored .Results Compared with group S , the serum BUN and Cr concentrations and pathological scores were significantly increased at T1 ,2 in I/R and P groups ,and the number of CD4+ CD25+ Foxp3+ Treg and CXCR3+ CD4+ CD25+ Foxp3+ Treg was increased at T2 in I/R group ( P<0.05) .Compared with group I/R ,the serum BUN and Cr concentrations and pathological scores were significantly increased at T2 ,and the number of CD4+ CD25+ Foxp3+ Treg and CXCR3+ CD4+ CD25+ Foxp3+ Treg was decreased at T2 in P group ( P<0.05 ) .Conclusion Up-regulation of CXCR3 is helpful in migration of Tregs into the renal tissues of mice with renal I/R injury .